AB8028

Anti-GFR alpha 3/GFRA3 antibody

Name: Anti-GFR alpha 3/GFRA3 antibody (ab8028) | Abcam
Brand: Abcam Limited
SKU: AB8028
Price: 515 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(10 Publications)

Rabbit Polyclonal GFR alpha 3/GFRA3 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 10 publications.

View Alternative Names

GDNF family receptor alpha-3, GDNF receptor alpha-3, GDNFR-alpha-3, GFR-alpha-3, Gfra3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3/GFRA3 with Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 5μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

ab8028 at 10μg/ml staining GFR alpha 3/GFRA3 in mouse heart tissue by IHC-P.

Unknown

Western blot - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

Western blot analysis of GFR alpha 3/GFRA3 in crude cell membrane fractions of
murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution.

All lanes:

Western blot - Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 1/500 dilution

Lane 1:

Crude cell membrane fractions of murine heart

Lane 2:

Crude cell membrane fractions of murine spleen

Lane 3:

Crude cell membrane fractions of murine kidney

Lane 4:

Crude cell membrane fractions of murine liver

Lane 5:

Crude cell membrane fractions of murine brain

Predicted band size: 45 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Product details

Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography DEAE-C

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for artemin (ARTN), a growth factor that supports the survival of sensory and sympathetic peripheral neurons. ARTN-binding leads to autophosphorylation and activation of the RET receptor.

See full target information Gfra3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 73:393-414 PubMed41015934

2025

Expression of Glial Cell Line-derived Neurotrophic Factor and Its Receptors in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jesper Dupont Ewald,Arnon Møldrup Knudsen,Helle Wohlleben,Lone Christiansen,Signe Regner Michaelsen,Atul Anand,Bjarne Winther Kristensen

International journal of cancer 148:1756-1767 PubMed33236361

2020

Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Jiao Li,Hao Li,Wu-Hu Zhang,Shuai-Shuai Xu,Wang Jiang,Shuo Li,He-Li Gao,Xuan Han,Hua-Xiang Xu,Chun-Tao Wu,Wen-Quan Wang,Xian-Jun Yu,Liang Liu

Acupuncture in medicine : journal of the British Medical Acupuncture Society 37:244-251 PubMed31188015

2019

Effect of moxibustion on the expression of GDNF and its receptor GFRα3 in the colon and spinal cord of rats with irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Qi,Huangan Wu,Xiaoming Jin,Duiyin Jin,Yuanyuan Wang,Cun Wang,Yanan Liu,Xiaomei Wang

PloS one 14:e0216527 PubMed31107888

2019

The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Frank L Rice,George Houk,James P Wymer,Sara J C Gosline,Justin Guinney,Jianqiang Wu,Nancy Ratner,Michael P Jankowski,Salvo La Rosa,Marilyn Dockum,James R Storey,Steven L Carroll,Phillip J Albrecht,Vincent M Riccardi

Cell 173:634-648.e12 PubMed29606356

2018

Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmei Han,Qiuyan Liu,Jin Hou,Yan Gu,Yi Zhang,Zhubo Chen,Jia Fan,Weiping Zhou,Shuangjian Qiu,Yonghong Zhang,Tao Dong,Ning Li,Zhengping Jiang,Ha Zhu,Qian Zhang,Yuanwu Ma,Lianfeng Zhang,Qingqing Wang,Yizhi Yu,Nan Li,Xuetao Cao

Proceedings of the National Academy of Sciences of 114:3927-3932 PubMed28356515

2017

Identification of targets of tumor suppressor microRNA-34a using a reporter library system.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiaki Ito,Atsushi Inoue,Timothy Seers,Yukari Hato,Arisa Igarashi,Tatsuya Toyama,Konstantin D Taganov,Mark P Boldin,Hiroshi Asahara

Journal of neuroscience research 92:1637-46 PubMed25044131

2014

Artemin augments survival and axon regeneration in axotomized retinal ganglion cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuko Omodaka,Takuji Kurimoto,Orie Nakamura,Kota Sato,Masayuki Yasuda,Yuji Tanaka,Noriko Himori,Yu Yokoyama,Toru Nakazawa

PloS one 7:e49328 PubMed23152897

2012

Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors.

Applications

IHC - Wmt

Species

Human

Kazuhiro Kawamura,Yuan Chen,Yimin Shu,Yuan Cheng,Jie Qiao,Barry Behr,Renee A Reijo Pera,Aaron J W Hsueh

Annals of surgery 244:274-81 PubMed16858191

2006

The neurotrophic factor artemin promotes pancreatic cancer invasion.

Applications

IHC-P, WB

Species

Human, Human

Güralp O Ceyhan,Nathalia A Giese,Mert Erkan,Annika G Kerscher,Moritz N Wente,Thomas Giese,Markus W Büchler,Helmut Friess

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 23:425-38 PubMed16002253

2005

Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Pina Serra,Marina Quartu,Francesca Mascia,Annalisa Manca,Marianna Boi,M Giuseppina Pisu,Maria Letizia Lai,Marina Del Fiacco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFR alpha 3/GFRA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

Western blot - Anti-GFR alpha 3/GFRA3 antibody (AB8028)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (10)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise