JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10551

Anti-GGA1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal GGA1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GGA1 aa 300 to C-terminus.

View Alternative Names

ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, GGA1

1 Images
Western blot - Anti-GGA1 antibody (AB10551)
  • WB

Unknown

Western blot - Anti-GGA1 antibody (AB10551)

The lysate prepared from HEK293 cells overexpressing YFP-GGA1 fusion protein. This accounts for the observed band of 100 kDa.

All lanes:

Western blot - Anti-GGA1 antibody (ab10551) at 1/1350 dilution

All lanes:

Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/mL

Secondary

All lanes:

Goat-anti Rabbit IgG

Predicted band size: 70 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GGA1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UJY5

Specificity

This affinity purified antibody is directed against human GGA1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA1 from bovine (87% homology), mouse (82% homology) and rat (76% homology) sources. The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. Cross reactivity with GGA1 homologues from other sources has not been determined.

style
left-column

Alternative Products

Primary Antibodies

AB170956

Anti-GGA1 antibody [EPR3014(2)]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (AB170956)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB34718

Anti-KDM5C / Jarid1C / SMCX antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KDM5C / Jarid1C / SMCX antibody (AB34718)

  • 4
  • 5
Primary Antibodies

AB157203

Anti-CDK5RAP3 antibody [EPR11294(B)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

  • 0
  • 0
Primary Antibodies

AB185557

Anti-GGA2 antibody [EPR14895]

RabMAb|Recombinant

Western blot - Anti-GGA2 antibody [EPR14895] (AB185557)

  • 0
  • 0
Primary Antibodies

AB227558

Anti-CCDC91 antibody

Western blot - Anti-CCDC91 antibody (AB227558)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This affinity purified antibody is directed against human GGA1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed : 11301005, PubMed : 15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed : 27901063). Required for targeting PKD1 : PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed : 15615712, PubMed : 15886016).
See full target information GGA1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Traffic (Copenhagen, Denmark) 8:1440-51 PubMed17666033

2007

The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes.

Applications

ICC/IF

Species

African green monkey

Stéphane Lefrançois,Peter J McCormick

The Journal of neuroscience : the official journal of the Society for Neuroscience 26:12838-46 PubMed17151287

2006

GGA1 is expressed in the human brain and affects the generation of amyloid beta-peptide.

Applications

WB

Species

Human

Tina Wahle,Dietmar R Thal,Magdalena Sastre,Andrea Rentmeister,Nenad Bogdanovic,Michael Famulok,Michael T Heneka,Jochen Walter
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com