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AB170956

Anti-GGA1 antibody [EPR3014(2)]

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(3 Publications)

Rabbit Recombinant Monoclonal GGA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, GGA1

3 Images
Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (AB170956)
  • IP

Supplier Data

Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (AB170956)

Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.

All lanes:

Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)

Predicted band size: 60 kDa,61 kDa,70 kDa

false

Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)
  • WB

Supplier Data

Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)

All lanes:

Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa

true

Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)
  • WB

Lab

Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)

Western blot : Rabbit Monoclonal[EPR3014(2)] to GGA1 ab170956 staining at 2 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 74 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in GGA1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 2 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

GGA1 knockout U-87 MG at 20 µg

Lane 3:

SH-SY5Y at 20 µg

Lane 4:

SK-OV-3 at 20 µg

Lane 5:

DU 145 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 74 kDa,36 kDa

false

  • Carrier free

    Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3014(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed : 11301005, PubMed : 15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed : 27901063). Required for targeting PKD1 : PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed : 15615712, PubMed : 15886016).
See full target information GGA1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 11:367-374 PubMed33206455

2021

Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting.

Applications

Unspecified application

Species

Unspecified reactive species

Balraj Doray,Lin Liu,Wang-Sik Lee,Benjamin C Jennings,Stuart Kornfeld

Molecular biology of the cell 31:27-44 PubMed31746668

2019

Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhi A Tan,Lou Fourriere,Jingqi Wang,Franck Perez,Gaelle Boncompain,Paul A Gleeson

Molecular biology of the cell 29:191-208 PubMed29142073

2017

GGA1 regulates signal-dependent sorting of BACE1 to recycling endosomes, which moderates Aβ production.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Hong Toh,Pei Zhi Cheryl Chia,Mohammed Iqbal Hossain,Paul A Gleeson
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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