Rabbit Recombinant Monoclonal GGA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.
Images
![Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (AB170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--immunoprecipitation-img53469.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (AB170956)")
![Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--western-blot-img99830.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)")
![Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--western-blot-img111489.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GGA1 antibody [EPR3014(2)] (AB170956)")
Publications
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- FEBS open bio 11:367-3742021Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBalraj Doray,Lin Liu,Wang-Sik Lee,Benjamin C Jennings,Stuart KornfeldPubMed 33206455
- Molecular biology of the cell 31:27-442019Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhi A Tan,Lou Fourriere,Jingqi Wang,Franck Perez,Gaelle Boncompain,Paul A GleesonPubMed 31746668
- Molecular biology of the cell 29:191-2082017GGA1 regulates signal-dependent sorting of BACE1 to recycling endosomes, which moderates Aβ production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Hong Toh,Pei Zhi Cheryl Chia,Mohammed Iqbal Hossain,Paul A GleesonPubMed 29142073
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005, PubMed:15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:27901063). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed:15615712, PubMed:15886016).
Alternative names
ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, GGA1
Recommended products
Rabbit Recombinant Monoclonal GGA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3014(2)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--immunoprecipitation-img53469.jpg "Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)")
Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.
All lanes: Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
Predicted band size: 60 kDa, 61 kDa, 70 kDa
![Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--western-blot-img99830.jpg "Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)")
Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: GGA1 knockout HAP1 cell lysate (20 μg)
Lane 3: Jurkat cell lysate (20 μg)
Lane 4: HeLa cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 2 μg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
Predicted band size: 70 kDa
![Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956), expandable thumbnail](https://content.abcam.com/products/images/gga1-antibody-epr30142-ab170956--western-blot-img111489.jpg "Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)")
Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
All lanes: Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
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