Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (AB249621)

This data was developed using ab170956, the same antibody clone in a different buffer formulation.

Western blot analysis on Immunoprecipitation pellet from Jurkat cells showing GGA1, using ab170956 at 1/10 dilution for IP.

All lanes:

Immunoprecipitation - Anti-GGA1 antibody [EPR3014(2)] (<a href='/en-us/products/primary-antibodies/gga1-antibody-epr30142-ab170956'>ab170956</a>)

Predicted band size: 60 kDa,61 kDa,70 kDa

false

• WB

Supplier Data

Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (AB249621)

This data was developed using ab170956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GGA1 antibody [EPR3014(2)] (<a href='/en-us/products/primary-antibodies/gga1-antibody-epr30142-ab170956'>ab170956</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa

true

• WB

Lab

Western blot - Anti-GGA1 antibody [EPR3014(2)] - BSA and Azide free (AB249621)

This data was developed using ab170956, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EPR3014(2)] to GGA1 ab170956 staining at 2 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 74 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in GGA1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-GGA1 antibody [EPR3014(2)] (<a href='/en-us/products/primary-antibodies/gga1-antibody-epr30142-ab170956'>ab170956</a>) at 2 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

GGA1 knockout U-87 MG at 20 µg

Lane 3:

SH-SY5Y at 20 µg

Lane 4:

SK-OV-3 at 20 µg

Lane 5:

DU 145 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 74 kDa,36 kDa

false