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AB180951

Anti-GGA3 antibody [EPR14340]

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(2 Publications)

Rabbit Recombinant Monoclonal GGA3 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0154, GGA3, ADP-ribosylation factor-binding protein GGA3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGA3 antibody [EPR14340] (AB180951)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGA3 antibody [EPR14340] (AB180951)

Immunohistochemical analysis of paraffin-embedded Human Iifiltrating duct carcinoma of breast tissue labeling GGA3 using ab180951 at a 1/50 dilution and counterstained with Hematoxylin. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-GGA3 antibody [EPR14340] (AB180951)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GGA3 antibody [EPR14340] (AB180951)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixedSH-SY5Y cells labeling GGA3 using ab180951 at a 1/20 dilutioin, orRabbit monoclonal IgG (isotype control). Goat anti rabbit IgG (FITC) at a 1/50 dilutionwasused as a secondary antibody.

Western blot - Anti-GGA3 antibody [EPR14340] (AB180951)
  • WB

Supplier Data

Western blot - Anti-GGA3 antibody [EPR14340] (AB180951)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GGA3 antibody [EPR14340] (ab180951) at 1/5000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 78 kDa

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  • Carrier free

    Anti-GGA3 antibody [EPR14340] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14340

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Western blot - Anti-GGA2 antibody [EPR14895] (AB185557)

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-CCDC91 antibody

Western blot - Anti-CCDC91 antibody (AB227558)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed : 11301005). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed : 26811329). nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels (PubMed : 15615712, PubMed : 17553422, PubMed : 20484053). Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation (PubMed : 15615712). Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination (PubMed : 20484053). Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (By similarity).
See full target information GGA3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:4337-4357 PubMed39242776

2024

HIV-1 Vpu induces neurotoxicity by promoting Caspase 3-dependent cleavage of TDP-43.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Yan Li,Huili Li,Haichen Zhang,Haoran Guo,Xiangyu Zheng,Xiao-Fang Yu,Wei Wei

Journal of ovarian research 17:31 PubMed38310251

2024

Hyperinsulinemia impairs decidualization via AKT-NR4A1 signaling: new insight into polycystic ovary syndrome (PCOS)-related infertility.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Xing Jiang,Wei-Jie Zhao,Hao-Ran Shen,Dan-Feng Du,Xue-Lian Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com