Rabbit Recombinant Monoclonal GGCT antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-GGCT antibody [EPR17107] (AB198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--western-blot-img13605.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GGCT antibody [EPR17107] (AB198503)")
![Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (AB198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunocytochemistry-immunofluorescence-img14429.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (AB198503)")
![Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (AB198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunocytochemistry-immunofluorescence-img13895.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (AB198503)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (AB198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112311.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (AB198503)")
Publications
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- Scientific reports 13:119222023Identification of c-Met as a novel target of γ-glutamylcyclotransferase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumiko Saito,Keiko Taniguchi,Hiromi Ii,Mano Horinaka,Susumu Kageyama,Susumu Nakata,Osamu Ukimura,Toshiyuki SakaiPubMed 37488242
- Endocrinology 163:2022MiR-205-5p/GGCT Attenuates Growth and Metastasis of Papillary Thyroid Cancer by Regulating CD44.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Ning Li,Hui-Min Zhang,Xing-Rui Li,Jun Wang,Tao Xu,Shu-Yu Li,Meng-Lu Dong,Ge Wang,Xiao-Qing Cui,Xue Yang,Yong-Lin Wu,Xing-Hua Liao,Ya-Ying DuPubMed 35213720
- British journal of haematology 195:267-2752021Ggct (γ-glutamyl cyclotransferase) plays an important role in erythrocyte antioxidant defense and red blood cell survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaoke He,Xiaoqin Sun,Shixiang Wang,Dongsheng Bai,Xiangyu Zhao,Ying Han,Piliang Hao,Xue-Song LiuPubMed 34409610
- International immunopharmacology 81:1060392019γ-Glutamyl cyclotransferase contributes to endometrial carcinoma malignant progression and upregulation of PD-L1 expression during activation of epithelial-mesenchymal transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengjie Xu,Ye Yang,Xiaoyun Wang,Xiangnan Liu,Chengjuan Jin,Mingjun Ma,Sufang Wu,Yanli LiPubMed 31757677
- iScience 19:256-2662019Ras Downstream Effector GGCT Alleviates Oncogenic Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaoke He,Shixiang Wang,Yuanyuan Shao,Jing Zhang,Xiaolin Wu,Yuxing Chen,Junhao Hu,Feng Zhang,Xue-Song LiuPubMed 31400748
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis (PubMed:18515354). Induces release of cytochrome c from mitochondria with resultant induction of apoptosis (PubMed:16765912).
Alternative names
C7orf24, CRF21, GGCT, Gamma-glutamylcyclotransferase, Cytochrome c-releasing factor 21
Recommended products
Rabbit Recombinant Monoclonal GGCT antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR17107
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-GGCT antibody [EPR17107] (ab198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--western-blot-img13605.jpg "Western blot - Anti-GGCT antibody [EPR17107] (ab198503)")
Western blot - Anti-GGCT antibody [EPR17107] (ab198503)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GGCT antibody [EPR17107] (ab198503) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Lane 2: 293 (Human embryonic kidney) whole cell lysate at 10 µg
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 4: Human fetal kidney lysate at 10 µg
Lane 5: Human fetal brain lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3min
![Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunocytochemistry-immunofluorescence-img14429.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)")
Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198503 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunocytochemistry-immunofluorescence-img13895.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)")
Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (ab198503)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GGCT with ab198503 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198503 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503), expandable thumbnail](https://content.abcam.com/products/images/ggct-antibody-epr17107-ab198503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112311.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGCT antibody [EPR17107] (ab198503)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GGCT with ab198503 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm and weak nuclear staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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