Rabbit Recombinant Monoclonal GGH antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-GGH antibody [EPR8014] (AB138495), expandable thumbnail](https://content.abcam.com/products/images/ggh-antibody-epr8014-ab138495--western-blot-img119521.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GGH antibody [EPR8014] (AB138495)")
![OI-RD Scanning - Anti-GGH antibody [EPR8014] (AB138495), expandable thumbnail](https://content.abcam.com/products/images/ggh-antibody-epr8014-ab138495--oi-rd-scanning-img95384.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-GGH antibody [EPR8014] (AB138495)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate (PubMed:11005824, PubMed:8816764). May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
Alternative names
Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase, GGH
Recommended products
Rabbit Recombinant Monoclonal GGH antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8014
- Purity
- Tissue culture supernatant
- Dissociation constant
- 3.78 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GGH antibody [EPR8014] (ab138495), expandable thumbnail](https://content.abcam.com/products/images/ggh-antibody-epr8014-ab138495--western-blot-img119521.jpg "Western blot - Anti-GGH antibody [EPR8014] (ab138495)")
Western blot - Anti-GGH antibody [EPR8014] (ab138495)
All lanes: Western blot - Anti-GGH antibody [EPR8014] (ab138495) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
![OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495), expandable thumbnail](https://content.abcam.com/products/images/ggh-antibody-epr8014-ab138495--oi-rd-scanning-img95384.jpg "OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)")
OI-RD Scanning - Anti-GGH antibody [EPR8014] (ab138495)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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