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Anti-GGT1/GGT antibody [1F9] (ab55138) is a mouse monoclonal antibody that is used to detect GGT1/GGT in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Over 10 publications

- Trusted since 2007


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [1F9] (AB55138), expandable thumbnail
  • Western blot - Anti-GGT1/GGT antibody [1F9] (AB55138), expandable thumbnail
  • Flow Cytometry - Anti-GGT1/GGT antibody [1F9] (AB55138), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GGT1/GGT antibody [1F9] (AB55138), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human GGT1 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link P19440

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Expected
Tested
Tested
Mouse
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4) (PubMed:17924658, PubMed:21447318, PubMed:27791009). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases (PubMed:27791009). In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed:17924658, PubMed:21447318, PubMed:7673200, PubMed:7759490, PubMed:8095045, PubMed:8827453). Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4. Isoform 3. Seems to be inactive.

Alternative names

CD224, GGT, GGT1, Glutathione hydrolase 1 proenzyme, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Leukotriene-C4 hydrolase, GGT 1

Recommended products

Anti-GGT1/GGT antibody [1F9] (ab55138) is a mouse monoclonal antibody that is used to detect GGT1/GGT in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Over 10 publications

- Trusted since 2007

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human GGT1 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link P19440
Carrier free
Yes
Clone number
1F9
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 20th Jan 2020. Lot numbers higher than GR3280625 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [1F9] (ab55138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [1F9] (ab55138)

    GGT1/GGT antibody (ab55138) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-GGT1/GGT antibody [1F9] (ab55138), expandable thumbnail

    Western blot - Anti-GGT1/GGT antibody [1F9] (ab55138)

    GGT1/GGT antibody (ab55138) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-GGT1/GGT antibody [1F9] (ab55138)

    Predicted band size: 61 kDa

  • Flow Cytometry - Anti-GGT1/GGT antibody [1F9] (ab55138), expandable thumbnail

    Flow Cytometry - Anti-GGT1/GGT antibody [1F9] (ab55138)

    Overlay histogram showing HEK293 cells stained with ab55138 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55138, 1μg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-GGT1/GGT antibody [1F9] (ab55138), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GGT1/GGT antibody [1F9] (ab55138)

    ICC/IF image of ab55138 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55138, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This image was generated using the ascites version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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