AB109427

Anti-GGT1/GGT antibody [EPR5288]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal GGT1/GGT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD224, GGT, GGT1, Glutathione hydrolase 1 proenzyme, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Leukotriene-C4 hydrolase, GGT 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

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Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 61 kDa

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Lab

Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Lane 3:

Human fetal kidney tissue lysate at 20 µg

Lane 4:

A549 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 50-60 kDa

false

Carrier free

Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5288

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4) (PubMed : 17924658, PubMed : 21447318, PubMed : 27791009). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases (PubMed : 27791009). In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed : 17924658, PubMed : 21447318, PubMed : 7673200, PubMed : 7759490, PubMed : 8095045, PubMed : 8827453). Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.. Isoform 3. Seems to be catalytically inactive.

See full target information GGT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 74:103234 PubMed38861834

2024

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Guo,Yumeng Li,Shiquan Wang,Yongheng Yang,Tiantian Xu,Jianshuai Zhao,Jin Wang,Wenqiang Zuo,Pengju Wang,Guangchao Zhao,Huaning Wang,Wugang Hou,Hailong Dong,Yanhui Cai

Frontiers in pharmacology 12:628651 PubMed33981220

2021

L. Regulates Glutathione Redox Stress and Normalizes Ggt1/Anpep Signaling to Alleviate OVX-Induced Kidney Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ru Liu,Ning-Juan Yang,Meng-Li Zhao,Zhi-Shu Tang,Jin-Ao Duan,Rui Zhou,Lin Chen,Jing Sun,Zhong-Xing Song,Jin-Hang Hu,Xin-Bo Shi

Molecular cancer research : MCR 17:1881-1892 PubMed31151999

2019

Gamma-Glutamyltransferase 1 Promotes Clear Cell Renal Cell Carcinoma Initiation and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ankita Bansal,Danielle J Sanchez,Vivek Nimgaonkar,David Sanchez,Romain Riscal,Nicolas Skuli,M Celeste Simon

Gut 62:1179-86 PubMed22698650

2012

Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Mitsunaga,Nobuyuki Kosaka,Peter L Choyke,Matthew R Young,Christopher R Dextras,Shakir M Saud,Nancy H Colburn,Masayo Sakabe,Tetsuo Nagano,Daisuke Asanuma,Yasuteru Urano,Hisataka Kobayashi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GGT1/GGT antibody [EPR5288]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise