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AB247856

Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free

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Rabbit Recombinant Monoclonal GGT1/GGT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CD224, GGT, GGT1, Glutathione hydrolase 1 proenzyme, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Leukotriene-C4 hydrolase, GGT 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)

This data was developed using ab109427, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)

This data was developed using ab109427, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)
  • WB

Unknown

Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)

This data was developed using ab109427, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (<a href='/en-us/products/primary-antibodies/ggt1-ggt-antibody-epr5288-ab109427'>ab109427</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 61 kDa

false

Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)
  • WB

Lab

Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (AB247856)

This data was developed using ab109427, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (<a href='/en-us/products/primary-antibodies/ggt1-ggt-antibody-epr5288-ab109427'>ab109427</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Lane 3:

Human fetal kidney tissue lysate at 20 µg

Lane 4:

A549 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 50-60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5288

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247856 is the carrier-free version of ab109427.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4) (PubMed : 17924658, PubMed : 21447318, PubMed : 27791009). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases (PubMed : 27791009). In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed : 17924658, PubMed : 21447318, PubMed : 7673200, PubMed : 7759490, PubMed : 8095045, PubMed : 8827453). Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.. Isoform 3. Seems to be catalytically inactive.
See full target information GGT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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