Anti-GGT5 antibody [EPR24682-102]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT5 antibody [EPR24682-102] (AB283267)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling GGT5 with ab283267 at 1/1000 (0.532 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Postive staining on the stroma cells in human lung carcinoma (PMID : 32640421). The section was incubated with ab283267 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT5 antibody [EPR24682-102] (AB283267)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling GGT5 with ab283267 at 1/1000 (0.532 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Postive staining on the stroma cells in human kidney(PMID : 25377544). The section was incubated with ab283267 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT5 antibody [EPR24682-102] (AB283267)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling GGT5 with ab283267 at 1/1000 (0.532 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Postive staining on the germinal center in human tonsil (PMID : 30842656). The section was incubated with ab283267 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-GGT5 antibody [EPR24682-102] (AB283267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 21447318, 1676842).

Exposure time : Lane 1, 2, 4 : 3.25 seconds Lane 3 : 10 seconds.

All lanes:

Western blot - Anti-GGT5 antibody [EPR24682-102] (ab283267) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human adrenal gland tissue lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-GGT5 antibody [EPR24682-102] (AB283267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 21447318, 1676842).

Negative control : IM-9 (PMID : 1676842).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 70 seconds.

All lanes:

Western blot - Anti-GGT5 antibody [EPR24682-102] (ab283267) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 62 kDa

Observed band size: 58 kDa

false