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AB273046

Anti-GGT7 antibody [EPR24550-76]

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(1 Publication)

Rabbit Recombinant Monoclonal GGT7 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GGTL3, GGTL5, GGT7, Glutathione hydrolase 7, Gamma-glutamyltransferase 7, Gamma-glutamyltransferase-like 3, Gamma-glutamyltransferase-like 5, Gamma-glutamyltranspeptidase 7, GGT 7

2 Images
Western blot - Anti-GGT7 antibody [EPR24550-76] (AB273046)
  • WB

Supplier Data

Western blot - Anti-GGT7 antibody [EPR24550-76] (AB273046)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046) at 1/1000 dilution

Lane 1:

Human hypothalamus lysate at 20 µg

Lane 2:

Human hypothalamus treated with PNGase F lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

U-87 MG treated with PNGase F lysate, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 70 kDa

Observed band size: 110 kDa,70 kDa,90 kDa

false

Exposure time: 3min

Western blot - Anti-GGT7 antibody [EPR24550-76] (AB273046)
  • WB

Supplier Data

Western blot - Anti-GGT7 antibody [EPR24550-76] (AB273046)

This blot was developed using a higher sensitivity ECL substrate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

U-2 OS transfected with GTT7-siRNA, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 70 kDa

Observed band size: 90 kDa

false

Exposure time: 3min

  • 805 Alexa Fluor® 790

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24550-76

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors.
See full target information GGT7

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:3913-3929 PubMed38911386

2024

TXNDC12 inhibits pancreatic tumor cells ferroptosis by regulating GSH/GGT7 and promotes its growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrong Xu,Yu Hei,Bobo Wang,Shuyue Tian,Xuanyu Chen,Jing Zhang,Fenghui Wang
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com