AB85104

Anti-Ghrelin Receptor/GHS-R antibody

Name: Anti-Ghrelin Receptor/GHS-R antibody (ab85104) | Abcam
Brand: Abcam Limited
SKU: AB85104
Rating: 4 (4 reviews)

(4 Reviews)

(13 Publications)

Rabbit Polyclonal Ghrelin Receptor/GHS-R antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 13 publications.

View Alternative Names

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, Ghrelin receptor, GHRP, GHSR

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin Receptor/GHS-R antibody (AB85104)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin Receptor/GHS-R antibody (AB85104)

ab85104 at 1/200 dilution staining Ghrelin Receptor/GHS-R in human fetal brain tissue section by immunohistochemistry (Formalin/ PFA fixed, paraffin-embedded tissue sections).

Unknown

Western blot - Anti-Ghrelin Receptor/GHS-R antibody (AB85104)

Rat liver lysate, 1 : 500 dilution.

All lanes:

Western blot - Anti-Ghrelin Receptor/GHS-R antibody (ab85104)

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Ghrelin Receptor also known as GHS-R or GHSR is a G protein-coupled receptor responsible for binding ghrelin the hunger hormone which is a ligand composed of ghrelin peptides. The receptor weighing approximately 44 kDa exhibits a chemical structure typical for G-protein-coupled receptors which enables signal transduction across cell membranes. The receptor is expressed extensively in the hypothalamus pituitary gland and other peripheral tissues including heart and adipose tissue indicating its widespread role in energy homeostasis.

Biological function summary

This target modulates several physiological processes. It notably has a significant influence on appetite regulation and energy balance reflecting its function as part of the larger neuroendocrine system. The Ghrelin Receptor does not form a larger protein complex but interacts directly with ghrelin analogues to mediate its effects. This interaction plays a critical role in stimulating growth hormone release influencing metabolic processes.

Pathways

The Ghrelin Receptor is integral to the growth hormone secretagogue pathway where it interacts closely with the growth hormone-releasing hormone receptor (GHRHR). This pathway exemplifies the receptor’s role in energy metabolism and regulation of body composition. In addition to this the receptor is involved in neuroendocrine signaling pathways impacting appetite and feeding behaviors by signaling to orexigenic neurons in the arcuate nucleus of the hypothalamus.

Research indicates that dysregulation of Ghrelin Receptor activity is linked to obesity and metabolic syndromes. Alterations in ghrelin signaling can lead to abnormal energy balance and fat storage. Moreover the receptor is associated with conditions like Prader-Willi Syndrome where ghrelin levels commonly increase exacerbating feeding behaviors. The Ghrelin Receptor's relationship with the growth hormone and its dynamic interaction with GHRHR also highlight its broader relevance in metabolic and growth-related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

G-protein-coupled receptor specific to ghrelin, an appetite-regulating peptide hormone commonly found in stomach (PubMed : 35027551, PubMed : 39833471). Upon activation, stimulates appetite and promotes growth hormone secretion (PubMed : 11322507, PubMed : 10604470, PubMed : 35027551, PubMed : 39833471). Also binds other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692, 429, MK-0677, adenosine) (PubMed : 11322507, PubMed : 10604470).

See full target information Growth hormone secretagogue receptor type 1

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Canadian journal of gastroenterology & hepatology 2023:1629777 PubMed38187112

2024

Ghrelin/GHSR Axis Induced M2 Macrophage and Alleviated Intestinal Barrier Dysfunction in a Sepsis Rat Model by Inactivating E2F1/NF-B Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhu,Zhimin Dou,Wei Wu,Qiliang Hou,Sen Wang,Ziqian Yuan,Bin Li,Jian Liu

Frontiers in endocrinology 13:932761 PubMed36387867

2022

Involvement of POMC neurons in LEAP2 regulation of food intake and body weight.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpin Chu,Hualing Peng,Nana Yu,Yuejin Zhang,Xueling Lin,Yisheng Lu

International journal of molecular sciences 23: PubMed35409338

2022

Ghrelin Represses Thymic Stromal Lymphopoietin Gene Expression through Activation of Glucocorticoid Receptor and Protein Kinase C Delta in Inflamed Skin Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hayan Jeong,Hyo-Jin Chong,Jangho So,Yejin Jo,Tae-Young Yune,Bong-Gun Ju

International journal of molecular sciences 23: PubMed35328456

2022

Effect of Bacterial Infection on Ghrelin Receptor Regulation in Periodontal Cells and Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Andressa V B Nogueira,Marjan Nokhbehsaim,Anna Damanaki,Sigrun Eick,Svenja Beisel-Memmert,Christian Kirschneck,Agnes Schröder,Thamiris Cirelli,Natalia D P Leguizamón,Joni A Cirelli,James Deschner

Molecular medicine reports 20:5050-5058 PubMed31638214

2019

Effects of β‑hydroxybutyric acid and ghrelin on the motility and inflammation of gastric antral smooth muscle cells involving the regulation of growth hormone secretagogue receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Hu,Li You,Changhua Hu,Juan Wu,Min Ai,Xiaoyan He,Wenjie Huang,Zonghui Wu

American journal of physiology. Gastrointestinal a 316:G453-G461 PubMed30702902

2019

Chronic alcohol exposure alters circulating insulin and ghrelin levels: role of ghrelin in hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Karuna Rasineni,Paul G Thomes,Jacy L Kubik,Edward N Harris,Kusum K Kharbanda,Carol A Casey

Cell communication and signaling : CCS 17:8 PubMed30683114

2019

Gut ghrelin regulates hepatic glucose production and insulin signaling via a gut-brain-liver pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lin,Zerong Liang,Liping He,Mengliu Yang,Dongfang Liu,Harvest F Gu,Hua Liu,Zhiming Zhu,Hongting Zheng,Ling Li,Gangyi Yang

Cell metabolism 27:461-469.e6 PubMed29233536

2017

LEAP2 Is an Endogenous Antagonist of the Ghrelin Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xuecai Ge,Hong Yang,Maria A Bednarek,Hadas Galon-Tilleman,Peirong Chen,Michael Chen,Joshua S Lichtman,Yan Wang,Olivier Dalmas,Yiyuan Yin,Hui Tian,Lutz Jermutus,Joseph Grimsby,Cristina M Rondinone,Anish Konkar,Daniel D Kaplan

Mediators of inflammation 2017:4916971 PubMed29317796

2017

Regulation of Ghrelin Receptor by Periodontal Bacteria and .

Applications

IHC

Species

Human

Marjan Nokhbehsaim,Anna Damanaki,Andressa Vilas Boas Nogueira,Sigrun Eick,Svenja Memmert,Xiaoyan Zhou,Shanika Nanayakkara,Werner Götz,Joni Augusto Cirelli,Andreas Jäger,James Deschner

Polish journal of veterinary sciences 20:467-475 PubMed29166288

2017

Spirulina Platensis Affects Factors Involved in Spermatogenesis and Increases Ghrelin Receptors in Testis Tissue of Rats Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

O B B Esener,E Gurel-Gurevin,B Isbilen-Basok,F Yigit,T Bilal,A Altiner,N Yilmazer,E I Armutak

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ghrelin Receptor/GHS-R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin Receptor/GHS-R antibody (AB85104)

Western blot - Anti-Ghrelin Receptor/GHS-R antibody (AB85104)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise