Anti-Giantin antibody [9B6] - Golgi Marker (ab37266) is a mouse monoclonal antibody detecting Giantin in ICC/IF. Suitable for Human.
- Over 60 publications
- Trusted since 2007
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img176892.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)")
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img44886.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)")
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img65454.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)")
Publications
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- Nature communications 14:73022023SARS-CoV-2 hijacks a cell damage response, which induces transcription of a more efficient Spike S-acyltransferase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco S Mesquita,Laurence Abrami,Lucie Bracq,Nattawadee Panyain,Vincent Mercier,Béatrice Kunz,Audrey Chuat,Joana Carlevaro-Fita,Didier Trono,F Gisou van der GootPubMed 37952051
- ACS omega 8:15406-154212023Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Danielewicz,Francesca Rosato,Jana Tomisch,Jonas Gräber,Birgit Wiltschi,Gerald Striedner,Winfried Römer,Juergen MairhoferPubMed 37151527
- Oncogene 42:1802-18202023Exosomal circSPIRE1 mediates glycosylation of E-cadherin to suppress metastasis of renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuannan Shu,Xuanxuan Lu,Yihui Pan,Junjie Cen,Kangbo Huang,Mi Zhou,Jun Lu,Jiaqi Dong,Hui Han,Wei Chen,Juan Lin,Junhang Luo,Jiaxing ZhangPubMed 37046045
- Molecular metabolism 70:1017002023A caveolin-1 dependent glucose-6-phosphatase trafficking contributes to hepatic glucose production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmandine Gautier-Stein,Julien Chilloux,Maud Soty,Bernard Thorens,Christophe Place,Carine Zitoun,Adeline Duchampt,Lorine Da Costa,Fabienne Rajas,Christophe Lamaze,Gilles MithieuxPubMed 36870604
- Traffic (Copenhagen, Denmark) 24:4-192022Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMai Ly Tran,Johanna Tüshaus,Yeongho Kim,Bulat R Ramazanov,Swathi Devireddy,Stefan F Lichtenthaler,Shawn M Ferguson,Julia von BlumePubMed 36398980
- Journal of cell science 135:2022MTCL2 promotes asymmetric microtubule organization by crosslinking microtubules on the Golgi membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRisa Matsuoka,Masateru Miki,Sonoko Mizuno,Yurina Ito,Chihiro Yamada,Atsushi SuzukiPubMed 35543016
- Scientific reports 12:50502022Loss of KAP3 decreases intercellular adhesion and impairs intracellular transport of laminin in signet ring cell carcinoma of the stomach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomohiro Soda,Yasuyuki Gen,Kei Terasaki,Naoto Iwai,Tomoko Kitaichi,Osamu Dohi,Hiroyoshi Taketani,Yuya Seko,Atsushi Umemura,Taichiro Nishikawa,Kanji Yamaguchi,Michihisa Moriguchi,Hideyuki Konishi,Yuji Naito,Yoshito Itoh,Kohichiroh YasuiPubMed 35322078
- Journal of cellular biochemistry 123:568-5802022Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew J Phillips,Marissa B Lobl,Yamnah A Hafeji,Hannah R Safranek,Ashley M Mohr,Justin L MottPubMed 34981854
- The EMBO journal 40:e1059902021NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCandice Kutchukian,Oscar Vivas,Maria Casas,Julia G Jones,Scott A Tiscione,Sergi Simó,Daniel S Ory,Rose E Dixon,Eamonn J DicksonPubMed 34019311
- Genome medicine 13:342021Biallelic variants in COPB1 cause a novel, severe intellectual disability syndrome with cataracts and variable microcephaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam L Macken,Annie Godwin,Gabrielle Wheway,Karen Stals,Liliya Nazlamova,Sian Ellard,Ahmed Alfares,Taghrid Aloraini,Lamia AlSubaie,Majid Alfadhel,Sulaiman Alajaji,Htoo A Wai,Jay Self,Andrew G L Douglas,Alexander P Kao,Matthew Guille,Diana BarallePubMed 33632302
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Recommended products
Anti-Giantin antibody [9B6] - Golgi Marker (ab37266) is a mouse monoclonal antibody detecting Giantin in ICC/IF. Suitable for Human.
- Over 60 publications
- Trusted since 2007
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 9B6
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Giantin antibody [9B6] - Golgi Marker (ab37266) is a mouse monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-Giantin [9B6] - Golgi Marker (ab37266) was first used in a scientific publication in 2007 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-Giantin [9B6] - Golgi Marker (ab37266) has over 10 independent reviews from customers.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Giantin also known as Giantin Golgi or Giantin protein is a large coiled-coil protein with a molecular mass of approximately 376 kDa. It localizes primarily to the cis/medial region of the Golgi apparatus serving as a Golgi marker. As a part of the structural framework of the Golgi it plays an important role in maintaining the integrity and architecture of the Golgi stacks. Giantin is highly expressed in a variety of cell types including those in the liver kidney and brain making it an important target in cell biology studies.
- Biological function summary
The Giantin protein functions as a scaffold that supports the Golgi apparatus’s dynamic organization. It is part of a larger complex that influences vesicular trafficking within the cell. This ability to interact with various vesicle coat proteins points to its important role in the sorting and transport of proteins and lipids through the secretory pathway. Besides Giantin is involved in the assembly of the Golgi matrix and influences its disassembly during mitosis suggesting a significant involvement in cell proliferation and division.
- Pathways
Giantin helps regulate intracellular transport pathways connecting the endoplasmic reticulum (ER) and Golgi as well as intra-Golgi trafficking. It interfaces with other significant proteins such as GM130 and GRASP65 which are involved in Golgi dynamics and structure. This protein also participates in the coordination of the secretory pathway important for the modification and sorting of proteins destined for the cell surface or lysosomes.
- Associated diseases and disorders
Giantin has been linked to conditions involving impaired protein glycosylation and Golgi-related transport defects. Notably defects in Giantin function are associated with connective tissue disorders and certain forms of Golgi dysfunctions like Congenital Disorders of Glycosylation (CDG). Additionally Giantin’s interactions with proteins such as Sec23/24 and Bet3 which are cargo-binding components of the COPII vesicle coat connect its functional disruption to diseases characterized by impaired vesicular trafficking and cellular secretion malfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img176892.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)")
Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
ab37266 staining Giantin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab37266 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img44886.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)")
Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266), expandable thumbnail](https://content.abcam.com/products/images/giantin-antibody-9b6-golgi-marker-ab37266--immunocytochemistry-immunofluorescence-img65454.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)")
This image is courtesy of a customer review submitted by Dr Kirk McManusImmunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
ab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.
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