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AB174655

Anti-Giantin antibody - Golgi Marker

4

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal Giantin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GOLGB1 aa 400-500.

View Alternative Names

Golgin subfamily B member 1, 372 kDa Golgi complex-associated protein, Giantin, Macrogolgin, GCP372, GOLGB1

2 Images
Western blot - Anti-Giantin antibody - Golgi Marker (AB174655)
  • WB

Supplier Data

Western blot - Anti-Giantin antibody - Golgi Marker (AB174655)

All lanes:

Western blot - Anti-Giantin antibody - Golgi Marker (ab174655) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 376 kDa

true

Exposure time: 10s

Immunoprecipitation - Anti-Giantin antibody - Golgi Marker (AB174655)
  • IP

Supplier Data

Immunoprecipitation - Anti-Giantin antibody - Golgi Marker (AB174655)

ab174655, at 1 μg/ml, labeling Giantin in 293T whole cell lysate immunoprecipitated using ab174655 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-Giantin antibody - Golgi Marker (ab174655)

Predicted band size: 376 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GOLGB1 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q14789

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab174655 is affinity purified using an epitope specific to Giantin immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Giantin also known as Giantin Golgi or Giantin protein is a large coiled-coil protein with a molecular mass of approximately 376 kDa. It localizes primarily to the cis/medial region of the Golgi apparatus serving as a Golgi marker. As a part of the structural framework of the Golgi it plays an important role in maintaining the integrity and architecture of the Golgi stacks. Giantin is highly expressed in a variety of cell types including those in the liver kidney and brain making it an important target in cell biology studies.
Biological function summary

The Giantin protein functions as a scaffold that supports the Golgi apparatus’s dynamic organization. It is part of a larger complex that influences vesicular trafficking within the cell. This ability to interact with various vesicle coat proteins points to its important role in the sorting and transport of proteins and lipids through the secretory pathway. Besides Giantin is involved in the assembly of the Golgi matrix and influences its disassembly during mitosis suggesting a significant involvement in cell proliferation and division.

Pathways

Giantin helps regulate intracellular transport pathways connecting the endoplasmic reticulum (ER) and Golgi as well as intra-Golgi trafficking. It interfaces with other significant proteins such as GM130 and GRASP65 which are involved in Golgi dynamics and structure. This protein also participates in the coordination of the secretory pathway important for the modification and sorting of proteins destined for the cell surface or lysosomes.

Giantin has been linked to conditions involving impaired protein glycosylation and Golgi-related transport defects. Notably defects in Giantin function are associated with connective tissue disorders and certain forms of Golgi dysfunctions like Congenital Disorders of Glycosylation (CDG). Additionally Giantin’s interactions with proteins such as Sec23/24 and Bet3 which are cargo-binding components of the COPII vesicle coat connect its functional disruption to diseases characterized by impaired vesicular trafficking and cellular secretion malfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May participate in forming intercisternal cross-bridges of the Golgi complex.
See full target information GOLGB1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14975 PubMed36056100

2022

Retro-2 alters Golgi structure.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Bopil Gim,Lianhui Zhu,Chuanting Tan,Yi Qian,Morven Graham,Xinran Liu,Intaek Lee

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

BMC biology 19:194 PubMed34493279

2021

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Yi Qian,Lianhui Zhu,Bopil Gim,Mengjing Bao,Jie Jia,Shuaiyang Jing,Yijing Wang,Chuanting Tan,Francesca Bottanelli,Pascal Ziltener,Sunkyu Choi,Piliang Hao,Intaek Lee

iScience 24:102901 PubMed34401678

2021

Use of dual-electron probes reveals the role of ferritin as an iron depot in erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Aronova,Seung-Jae Noh,Guofeng Zhang,Colleen Byrnes,Emily Riehm Meier,Young C Kim,Richard D Leapman

Drug metabolism and disposition: the biological fate of chemicals 49:361-368 PubMed33674270

2021

The Long Noncoding RNA Hepatocyte Nuclear Factor 4 Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Wang,Shitong Chen,Yiting Wang,Xiaofei Wang,Liang Yan,Kun Yang,Xiao-Bo Zhong,Shengna Han,Lirong Zhang
View all publications
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