Rabbit Polyclonal Giantin antibody. Golgi marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 30 publications.
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- Nature communications 14:77552023Molecular basis for the catalytic mechanism of human neutral sphingomyelinases 1 (hSMPD2).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingbo Yi,Boya Qi,Jian Yin,Ruochong Li,Xudong Chen,Junhan Hu,Guohui Li,Sensen Zhang,Yuebin Zhang,Maojun YangPubMed 38012235
- Nature communications 14:9482023Identification of global inhibitors of cellular glycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Madriz Sørensen,Christian Büll,Thomas D Madsen,Erandi Lira-Navarrete,Thomas Mandel Clausen,Alex E Clark,Aaron F Garretson,Richard Karlsson,Johan F A Pijnenborg,Xin Yin,Rebecca L Miller,Sumit K Chanda,Thomas J Boltje,Katrine T Schjoldager,Sergey Y Vakhrushev,Adnan Halim,Jeffrey D Esko,Aaron F Carlin,Ramon Hurtado-Guerrero,Roberto Weigert,Henrik Clausen,Yoshiki NarimatsuPubMed 36804936
- Methods in molecular biology (Clifton, N.J.) 2557:519-5282022Studying the Role of Lipid Geometry in COPI Vesicle Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyewon Choi,Kunyou Park,Victor W Hsu,Seung-Yeol ParkPubMed 36512234
- Cell reports 41:1116532022USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Hertel,Ludovico Martins Alves,Henrik Dutz,Georg Tascher,Florian Bonn,Manuel Kaulich,Ivan Dikic,Stefan Eimer,Florian Steinberg,Anja BremmPubMed 36476874
- Frontiers in cell and developmental biology 10:10112212022A novel diG motif in ORF3a protein of SARS-Cov-2 for intracellular transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuth Cruz-Cosme,Jiantao Zhang,Dongxiao Liu,Vidhyanand Mahase,Bhargava Teja Sallapalli,Peixi Chang,Yanjin Zhang,Shaolei Teng,Richard Y Zhao,Qiyi TangPubMed 36506095
- Frontiers in genetics 13:10348892022G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Liu,Shaoping Tian,Tianyu Lin,Xin He,Justin Eze Ideozu,Rui Wang,Yong Wang,Dan Yue,Hua GengPubMed 36330442
- PLoS pathogens 18:e10108922022Requirement of a functional ion channel for Sindbis virus glycoprotein transport, CPV-II formation, and efficient virus budding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeinab Elmasri,Vashi Negi,Richard J Kuhn,Joyce JosePubMed 36191050
- Cell reports 40:1111952022Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Luo,Qiangqiang Liu,Hongcheng Cheng,Jiale Wang,Tian Zhao,Jiaojiao Zhang,Chenglong Mu,Yuanyuan Meng,Linbo Chen,Changqian Zhou,Hong Lei,Jianyu Yang,Guo Chen,Yanjun Li,Leiting Pan,Quan Chen,Yushan ZhuPubMed 35977480
- Biomedicines 10:2022E-Cadherin Modulation and Inter-Cellular Trafficking in Tubular Gastric Adenocarcinoma: A High-Resolution Microscopy Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlona Mihaela Liliac,Bogdan Silviu Ungureanu,Claudiu Mărgăritescu,Victor Mihai Sacerdoțianu,Adrian Săftoiu,Laurențiu Mogoantă,Emil Moraru,Daniel PiriciPubMed 35203558
- iScience 25:1036432022Specific motifs mediate post-synaptic and surface transport of G protein-coupled receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Xu,Zhe Wei,Guangyu WuPubMed 35024582
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May participate in forming intercisternal cross-bridges of the Golgi complex.
Alternative names
Golgin subfamily B member 1, 372 kDa Golgi complex-associated protein, Giantin, Macrogolgin, GCP372, GOLGB1
Recommended products
Rabbit Polyclonal Giantin antibody. Golgi marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 30 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Giantin also known as Giantin Golgi or Giantin protein is a large coiled-coil protein with a molecular mass of approximately 376 kDa. It localizes primarily to the cis/medial region of the Golgi apparatus serving as a Golgi marker. As a part of the structural framework of the Golgi it plays an important role in maintaining the integrity and architecture of the Golgi stacks. Giantin is highly expressed in a variety of cell types including those in the liver kidney and brain making it an important target in cell biology studies.
- Biological function summary
The Giantin protein functions as a scaffold that supports the Golgi apparatus’s dynamic organization. It is part of a larger complex that influences vesicular trafficking within the cell. This ability to interact with various vesicle coat proteins points to its important role in the sorting and transport of proteins and lipids through the secretory pathway. Besides Giantin is involved in the assembly of the Golgi matrix and influences its disassembly during mitosis suggesting a significant involvement in cell proliferation and division.
- Pathways
Giantin helps regulate intracellular transport pathways connecting the endoplasmic reticulum (ER) and Golgi as well as intra-Golgi trafficking. It interfaces with other significant proteins such as GM130 and GRASP65 which are involved in Golgi dynamics and structure. This protein also participates in the coordination of the secretory pathway important for the modification and sorting of proteins destined for the cell surface or lysosomes.
- Associated diseases and disorders
Giantin has been linked to conditions involving impaired protein glycosylation and Golgi-related transport defects. Notably defects in Giantin function are associated with connective tissue disorders and certain forms of Golgi dysfunctions like Congenital Disorders of Glycosylation (CDG). Additionally Giantin’s interactions with proteins such as Sec23/24 and Bet3 which are cargo-binding components of the COPII vesicle coat connect its functional disruption to diseases characterized by impaired vesicular trafficking and cellular secretion malfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody - Golgi Marker (ab80864)
ab80864 staining Giantin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab80864 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Giantin antibody - Golgi Marker (ab80864)
Lanes 1 - 2: Merged signal (red and green). Green - ab80864 observed at 377kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130kDa.ab80864 was shown to recognize GOLGB1 in wild-type HAP1 cells as signal was lost at the expected MW in GOLGB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GOLGB1 knockout samples were subjected to SDS-PAGE. ab80864 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Giantin antibody - Golgi Marker (ab80864) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: GOLGB1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 376 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Giantin antibody - Golgi Marker (ab80864)
IHC image of ab80864 staining in human kidney formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80864, 0.1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Giantin antibody - Golgi Marker (ab80864)
ab80864 staining Giantin in WIF-B cells (hepatoma-derived hybrid cell line) by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with formaldehyde; permeabilized with 0.2% Triton X-100 and blocked with 1% Donkey serum in 0.1% PBST for 1 hour at 22°C. Samples were incubated at 1/25 dilution for 3 hours at 22°C. An Alexa Fluor® 594 Donkey anti rabbit was used as the secondary antibody at 1/200 dilution.
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