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AB313998

Anti-Giardia lamblia Antibody [1F7]

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Mouse Monoclonal Giardia lamblia antibody. Suitable for ICC/IF and reacts with Giardia lamblia samples.

View Alternative Names

G lamblia, Giardia intestinalis

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Giardia lamblia Antibody [1F7] (AB313998)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Giardia lamblia Antibody [1F7] (AB313998)

Immunofluorescent analysis of Anti-Giardia lamblia Antibody [1F7] (ab313998). Giardia lamblia cysts were deactivated with 5% formalin. The primary antibody, ab313998, was used at 37°C for 30 mins, followed by the secondary antibody Goat anti-Mouse IgG (H+L), FITC conjugated at 1 µg/ml at 37°C for 30 mins (Green). Nuclear DNA was labelled with DAPI (shown in blue).

Immunocytochemistry/ Immunofluorescence - Anti-Giardia lamblia Antibody [1F7] (AB313998)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Giardia lamblia Antibody [1F7] (AB313998)

Negative control : Parallel staining with secondary antibody, Goat anti-Mouse IgG (H+L), FITC conjugated, only.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F7

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Giardia lamblia

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Giardia lamblia": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.097% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Giardia lamblia often called Giardia is a flagellated protozoan parasite and its mechanical function is important in understanding giardiasis the disease it causes. Its trophozoite form which is around 10-20 µm long attaches to the intestinal epithelium of the host leading to infection. The organism uses its adhesive disc to interact with the host. The parasite is able to express various surface proteins to adapt to the host’s immune system; among these variant-specific surface proteins (VSPs) play a significant role in its survival and virulence.
Biological function summary

Giardia lamblia causes diarrhea and malnutrition due to malabsorption in the intestines. The organism disrupts the normal epithelial barrier which results in impaired digestion and nutrient uptake. It is not part of a complex but does produce cysts that resist harsh environmental conditions outside the host. These cysts facilitate transmission and protect the parasite until it reaches a new host. The detected antigens in the stool play an important role in diagnosis such as using tests like antigardia and giardia ELISA.

Pathways

The interaction of Giardia lamblia with the intestinal cells involves several significant biological processes. It disrupts host signaling pathways associated with tight junctions such as the actin cytoskeleton pathway. Related proteins like myosin light chain kinase (MLCK) become activated leading to alteration of epithelial barrier functions. This affects the integrity of intestinal tight junctions causing permeability issues and contributing to diarrhea.

Giardia lamblia is closely connected with giardiasis characterized by symptoms like diarrhea weight loss and abdominal discomfort. The disruption of the intestinal epithelial barrier by Giardia relates to another protein zonulin which is associated with increased intestinal permeability. Although usually self-limiting giardiasis can have significant impacts especially in children and immunocompromised individuals triggering other gastrointestinal disorders if not properly addressed.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Giardia lamblia
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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