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Rabbit Polyclonal Gigaxonin antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GAN aa 50-100.

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Images

Immunoprecipitation - Anti-Gigaxonin antibody (AB101990), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human GAN aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2C0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Chimpanzee
Predicted
Cow
Predicted
Dog
Predicted
Gorilla
Predicted
Guinea pig
Predicted
Orangutan
Predicted
Pig
Predicted
Rabbit
Predicted
Rhesus monkey
Predicted
Turkey
Predicted

Tested
Tested

Species

Human

Dilution info

2.00000-5.00000 µg/mg of lysate

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Chimpanzee

Dilution info

-

Notes

-

Target data

Function

Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of TBCB. Controls degradation of MAP1B and MAP1S, and is critical for neuronal maintenance and survival.

Alternative names

Recommended products

Rabbit Polyclonal Gigaxonin antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GAN aa 50-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human GAN aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2C0
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Gigaxonin also known as GAN or KLHL16 is a cytoskeletal protein with a mass of approximately 69 kDa. It functions as an E3 ubiquitin ligase adapter and is part of the BTB-Kelch protein family. By regulating protein degradation gigaxonin maintains cellular homeostasis. This protein is especially expressed in neural tissues including the brain and spinal cord. Through its role in controlling the turnover of intermediate filament proteins like vimentin gigaxonin plays an essential part in maintaining the structural integrity of cells.

Biological function summary

Gigaxonin plays an important role in the nervous system and supports healthy neuronal function. It functions in conjunction with other components as part of the ubiquitin-proteasome system complex ensuring the timely degradation of specific proteins. This collaboration ensures the removal of misfolded or damaged proteins preventing neuronal dysfunction. The regulation of intermediate filaments and microtubules by gigaxonin is also important for cellular architecture and intracellular transport which are key for proper neuronal activity.

Pathways

The protein is integral to both the cytoskeleton organization and protein degradation pathways. In these pathways gigaxonin interacts with proteins like tubulin and peripherin influencing microtubule dynamics and neuronal differentiation. By overseeing the balance between synthesis and degradation of cytoskeletal elements gigaxonin ensures neuron integrity and function. These pathways involving gigaxonin are needed for protein quality control and overall cellular health.

Associated diseases and disorders

Gigaxonin mutations are linked to giant axonal neuropathy (GAN) a rare inherited neurodegenerative condition characterized by abnormally large axons and motor and sensory deficits. Aberrations in gigaxonin impair its ability to mediate the degradation of proteins such as neurofilaments leading to their accumulation. The linkage to GAN emphasizes the protein's role in maintaining neural health. Additionally abnormalities in gigaxonin function have implications for neurodegenerative diseases where intermediate filament accumulation presents a common feature.

Product promise

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1 product image

  • Immunoprecipitation - Anti-Gigaxonin antibody (ab101990), expandable thumbnail

    Immunoprecipitation - Anti-Gigaxonin antibody (ab101990)

    Immunoprecipitation of 1mg HeLa cell lysate using 3ug of antibody, 20% loaded on to Western blot and probed with 1ug/ml ab101991 (anti-gigaxonin antibody with an epitope downstream of ab101990). Lane 1: IP using ab101990. Lane2: IP using ab101991. Lane 3: IP using control IgG.

    All lanes: Immunoprecipitation - Anti-Gigaxonin antibody (ab101990)

    Predicted band size: 68 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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