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AB101990

Anti-Gigaxonin antibody

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(1 Publication)

Rabbit Polyclonal Gigaxonin antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GAN aa 50-100.

View Alternative Names

GAN1, KLHL16, GAN, Gigaxonin, Kelch-like protein 16

1 Images
Immunoprecipitation - Anti-Gigaxonin antibody (AB101990)
  • IP

Unknown

Immunoprecipitation - Anti-Gigaxonin antibody (AB101990)

Immunoprecipitation of 1mg HeLa cell lysate using 3ug of antibody, 20% loaded on to Western blot and probed with 1ug/ml ab101991 (anti-gigaxonin antibody with an epitope downstream of ab101990). Lane 1 : IP using ab101990. Lane2 : IP using ab101991. Lane 3 : IP using control IgG.

All lanes:

Immunoprecipitation - Anti-Gigaxonin antibody (ab101990)

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human GAN aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H2C0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gigaxonin also known as GAN or KLHL16 is a cytoskeletal protein with a mass of approximately 69 kDa. It functions as an E3 ubiquitin ligase adapter and is part of the BTB-Kelch protein family. By regulating protein degradation gigaxonin maintains cellular homeostasis. This protein is especially expressed in neural tissues including the brain and spinal cord. Through its role in controlling the turnover of intermediate filament proteins like vimentin gigaxonin plays an essential part in maintaining the structural integrity of cells.
Biological function summary

Gigaxonin plays an important role in the nervous system and supports healthy neuronal function. It functions in conjunction with other components as part of the ubiquitin-proteasome system complex ensuring the timely degradation of specific proteins. This collaboration ensures the removal of misfolded or damaged proteins preventing neuronal dysfunction. The regulation of intermediate filaments and microtubules by gigaxonin is also important for cellular architecture and intracellular transport which are key for proper neuronal activity.

Pathways

The protein is integral to both the cytoskeleton organization and protein degradation pathways. In these pathways gigaxonin interacts with proteins like tubulin and peripherin influencing microtubule dynamics and neuronal differentiation. By overseeing the balance between synthesis and degradation of cytoskeletal elements gigaxonin ensures neuron integrity and function. These pathways involving gigaxonin are needed for protein quality control and overall cellular health.

Gigaxonin mutations are linked to giant axonal neuropathy (GAN) a rare inherited neurodegenerative condition characterized by abnormally large axons and motor and sensory deficits. Aberrations in gigaxonin impair its ability to mediate the degradation of proteins such as neurofilaments leading to their accumulation. The linkage to GAN emphasizes the protein's role in maintaining neural health. Additionally abnormalities in gigaxonin function have implications for neurodegenerative diseases where intermediate filament accumulation presents a common feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of TBCB. Controls degradation of MAP1B and MAP1S, and is critical for neuronal maintenance and survival.
See full target information GAN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 326:C214-C228 PubMed38073486

2023

Parkin-mediated mitophagy is a potential treatment for oxaliplatin-induced peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhao,Te Zhang,Jiannan Li,Longyun Li,Peng Chen,Chunlu Zhang,Kai Li,Cancan Cui
View all publications
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