Rabbit Polyclonal Gigaxonin antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GAN aa 50-100.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate
Liquid
Polyclonal
IP | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Chicken | Predicted |
Chimpanzee | Predicted |
Cow | Predicted |
Dog | Predicted |
Gorilla | Predicted |
Guinea pig | Predicted |
Orangutan | Predicted |
Pig | Predicted |
Rabbit | Predicted |
Rhesus monkey | Predicted |
Turkey | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Chimpanzee | Dilution info - | Notes - |
Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of TBCB. Controls degradation of MAP1B and MAP1S, and is critical for neuronal maintenance and survival.
GAN1, KLHL16, GAN, Gigaxonin, Kelch-like protein 16
Rabbit Polyclonal Gigaxonin antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GAN aa 50-100.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Gigaxonin also known as GAN or KLHL16 is a cytoskeletal protein with a mass of approximately 69 kDa. It functions as an E3 ubiquitin ligase adapter and is part of the BTB-Kelch protein family. By regulating protein degradation gigaxonin maintains cellular homeostasis. This protein is especially expressed in neural tissues including the brain and spinal cord. Through its role in controlling the turnover of intermediate filament proteins like vimentin gigaxonin plays an essential part in maintaining the structural integrity of cells.
Gigaxonin plays an important role in the nervous system and supports healthy neuronal function. It functions in conjunction with other components as part of the ubiquitin-proteasome system complex ensuring the timely degradation of specific proteins. This collaboration ensures the removal of misfolded or damaged proteins preventing neuronal dysfunction. The regulation of intermediate filaments and microtubules by gigaxonin is also important for cellular architecture and intracellular transport which are key for proper neuronal activity.
The protein is integral to both the cytoskeleton organization and protein degradation pathways. In these pathways gigaxonin interacts with proteins like tubulin and peripherin influencing microtubule dynamics and neuronal differentiation. By overseeing the balance between synthesis and degradation of cytoskeletal elements gigaxonin ensures neuron integrity and function. These pathways involving gigaxonin are needed for protein quality control and overall cellular health.
Gigaxonin mutations are linked to giant axonal neuropathy (GAN) a rare inherited neurodegenerative condition characterized by abnormally large axons and motor and sensory deficits. Aberrations in gigaxonin impair its ability to mediate the degradation of proteins such as neurofilaments leading to their accumulation. The linkage to GAN emphasizes the protein's role in maintaining neural health. Additionally abnormalities in gigaxonin function have implications for neurodegenerative diseases where intermediate filament accumulation presents a common feature.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunoprecipitation of 1mg HeLa cell lysate using 3ug of antibody, 20% loaded on to Western blot and probed with 1ug/ml ab101991 (anti-gigaxonin antibody with an epitope downstream of ab101990). Lane 1: IP using ab101990. Lane2: IP using ab101991. Lane 3: IP using control IgG.
All lanes: Immunoprecipitation - Anti-Gigaxonin antibody (ab101990)
Predicted band size: 68 kDa
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