AB177509

Anti-GIGYF1 antibody

Name: Anti-GIGYF1 antibody (ab177509) | Abcam
Brand: Abcam Limited
SKU: AB177509
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GIGYF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GIGYF1 aa 350-400.

View Alternative Names

CDS2, PERQ1, PP3360, GIGYF1, GRB10-interacting GYF protein 1, PERQ amino acid-rich with GYF domain-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-GIGYF1 antibody (AB177509)

Detection of GIGYF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177509 at 6 μg/mg lysate for IP (Lane 1). For WB detection, ab177509 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-GIGYF1 antibody (ab177509)

Predicted band size: 115 kDa

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Supplier Data

Western blot - Anti-GIGYF1 antibody (AB177509)

All lanes:

Western blot - Anti-GIGYF1 antibody (ab177509) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 115 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GIGYF1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

O75420

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab177509 was affinity purified using an epitope specific to GIGYF1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GIGYF1 also known as Grb10 Interacting GYF Protein 1 is a protein involved in cellular signaling. The protein has a mass of approximately 140 kDa. It is expressed widely in various tissues with significant expression in the brain pancreas and testis. GIGYF1 contains the GYF domain which enables interaction with other proteins involved in signal transduction.

Biological function summary

GIGYF1 interacts with the insulin receptor pathway playing a role in growth factor signaling. It forms a complex with Grb10 working together to affect cellular responses to insulin. This protein is essential for growth regulation and metabolic processes offering a bridge between external signals and internal cellular mechanisms. GIGYF1's interaction with insulin signaling makes it a significant player in maintaining energy homeostasis.

Pathways

GIGYF1 impacts both the insulin and IGF signaling pathways which are important for regulating metabolism and growth. It interacts closely with proteins such as mTOR and Akt influencing cell survival and growth processes. GIGYF1 acts as a mediator in transmitting signals from these pathways affecting overall cellular responses to insulin.

GIGYF1 has been linked to diabetes and some forms of cancer. Alterations in GIGYF1 expression may disrupt normal insulin signaling contributing to insulin resistance associated with diabetes. In cancer its interaction with mTOR could influence cancer cell growth and survival given mTOR’s role in cell proliferation and metabolism. Understanding GIGYF1's role in these disorders could provide insights into disease mechanisms and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1 (By similarity).

See full target information GIGYF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

Journal of oncology 2022:3982539 PubMed35578600

2022

A Pan-Cancer Analysis of UBE2S in Tumorigenesis, Prognosis, Pathway, Immune Infiltration and Evasion, and Therapy Response from an Immune-Oncology Perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Haili Bao,Yi Luo,Guoshan Ding,Zhiren Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GIGYF1 antibody

Immunoprecipitation - Anti-GIGYF1 antibody (AB177509)

Western blot - Anti-GIGYF1 antibody (AB177509)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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