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AB232915

Anti-GILT antibody

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(3 Publications)

Rabbit Polyclonal GILT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFI30.

View Alternative Names

GILT, IP30, IFI30, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, Legumaturain

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human stomach tissue stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human lung tissue stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human glioma tissue stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human prostate cancer stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human colorectal cancer tissue stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GILT antibody (AB232915)
  • WB

Supplier Data

Western blot - Anti-GILT antibody (AB232915)

All lanes:

Western blot - Anti-GILT antibody (ab232915) at 3 µg/mL

All lanes:

Raji (human Burkitt's lymphoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GILT antibody (AB232915)

Paraffin-embedded human prostate tissue stained for GILT using ab232915 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-GILT antibody (AB232915)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GILT antibody (AB232915)

U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GILT (green) using ab232915 at 20 μg/ml in ICC/IF, followed by FITC staining.

Western blot - Anti-GILT antibody (AB232915)
  • WB

Supplier Data

Western blot - Anti-GILT antibody (AB232915)

All lanes:

Western blot - Anti-GILT antibody (ab232915) at 3 µg/mL

All lanes:

Recombinant human GILT protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFI30.

P13284

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Complementary Products

Proteins & Peptides

AB134622

Recombinant Human GILT protein

SDS-PAGE - Recombinant Human GILT protein (AB134622)

  • 0
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Primary Antibodies

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Flow Cytometry (Intracellular) - Anti-IFRD1 antibody [EPR28794-89] - BSA and Azide free (AB318975)

  • 0
  • 0
Primary Antibodies

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Anti-PCCB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

  • 0
  • 0
Primary Antibodies

AB70319

Anti-UBAP2L antibody

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  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab232915 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma-interferon-inducible lysosomal thiol reductase often called GILT is a thiol reductase enzyme with a mass of about 35 kDa. GILT is expressed predominantly in antigen-presenting cells where it resides in the lysosomes. Mechanically GILT catalyzes the reduction of disulfide bonds in proteins which enables proteins to unfold under mild acidic conditions found in lysosomal compartments. GILT activity requires the catalytic cysteine residue which is essential for its function as a reductase.
Biological function summary

GILT contributes to the processing and presentation of antigens. By reducing disulfide bonds in endocytosed proteins GILT prepares them for subsequent degradation by lysosomal proteases. This function means it plays a significant role in the immune response as it aids the generation of peptides presented on major histocompatibility complex (MHC) class II molecules. GILT is not known to be part of a larger protein complex but interacts with other components within the lysosome to accomplish its task.

Pathways

GILT is integral to the antigen processing and presentation pathway mediated by MHC class II molecules. This pathway is critical for the initiation of immune responses and involves collaboration with other proteins such as cathepsins. The reduction of disulfide bonds by GILT allows cathepsins to efficiently degrade proteins into peptide fragments. These peptides then bind to MHC class II molecules which are presented on the cell surface for recognition by CD4+ T cells.

Altered GILT function has been linked to autoimmune diseases and cancer. In certain autoimmune disorders dysregulation of GILT can lead to improper antigen presentation contributing to the body's immune response against its own tissues. In cancer some tumors may downregulate GILT to escape immune surveillance by reducing antigen presentation. Interactions between GILT and other proteins such as MHC class II facilitate these pathological processes highlighting the importance of GILT in maintaining immune system balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction (PubMed : 10639150). Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation (PubMed : 10639150). Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction (PubMed : 10852914). Dithiothreitol, cysteine, and cysteinyl glycine, but not glutathione, are capable of regenerating active precursor and mature IFI30 in vitro (PubMed : 10639150, PubMed : 10852914).
See full target information IFI30
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Folia histochemica et cytobiologica 63:11-27 PubMed40152123

2025

Inhibition of STAT1 alleviates oxidative damage in retinal pigment epithelial cells and exhibits neuroprotective effects in autoimmune optic neuritis by upregulating IFI30 lysosomal thiol reductase.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Ma,Jiajia Yuan

International journal of molecular sciences 24: PubMed36982231

2023

Early Pregnancy Regulates Expression of IkappaB Family in Ovine Spleen and Lymph Nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Shengya Fang,Chunjiang Cai,Ying Bai,Leying Zhang,Ling Yang

OncoTargets and therapy 13:1129-1143 PubMed32103982

2020

IFI30 Is a Novel Immune-Related Target with Predicting Value of Prognosis and Treatment Response in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhu,Xin Chen,Gefei Guan,Cunyi Zou,Qing Guo,Peng Cheng,Wen Cheng,Anhua Wu
View all publications
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Product promise

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