AB197123

Anti-GINS2 antibody

Name: Anti-GINS2 antibody (ab197123) | Abcam
Brand: Abcam Limited
SKU: AB197123

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(9 Publications)

Rabbit Polyclonal GINS2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GINS2 aa 1 to C-terminus.

View Alternative Names

PSF2, CGI-122, DC5, HSPC037, GINS2, DNA replication complex GINS protein PSF2, GINS complex subunit 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GINS2 antibody (AB197123)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GINS2 antibody (AB197123)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human fetal stomach tissue labeling GINS2 with ab197123 at 1/100.

Supplier Data

Western blot - Anti-GINS2 antibody (AB197123)

All lanes:

Western blot - Anti-GINS2 antibody (ab197123) at 1/500 dilution

All lanes:

Human fetal muscle lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GINS2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y248

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GINS2 protein also known as PSF2 is a part of the GINS complex essential for DNA replication initiation and synthesis. It has a mass of approximately 21 kilodaltons. This protein is expressed in various tissues particularly in those with high proliferative capacity such as the thymus and testis. GINS2 plays a fundamental role in the assembly of the pre-replication complex working alongside other core replication factors.

Biological function summary

GINS2 functions as a part of the tetrameric GINS complex which includes the proteins GINS1 (Sld5) GINS3 (Psf3) and GINS4 (Psf1). This complex is indispensable for the progression of the DNA replication fork and interacts with other replication machinery components like CDC45 and Mcm proteins. The collaboration of these proteins ensures successful DNA unwinding and synthesis demonstrating the critical role of GINS2 in cell proliferation and genomic stability.

Pathways

GINS2 is important in the DNA replication pathway and the cell cycle control mechanism. It closely interacts with proteins like CDC45 and Mcm2-7 facilitating the replication fork formation and movement during the S phase of the cell cycle. These interactions integrate GINS2 into the larger network that orchestrates DNA replication ensuring precise duplication of the genome before cell division.

GINS2 shows a connection to cancer and genomic instability disorders. Overexpression or dysfunction of GINS2 can result in unregulated cell proliferation a characteristic often observed in various cancers. The protein associates with the Mcm complex and CDC45 contributing to unwarranted cell cycle progression and tumor development. Understanding the GINS2 role in these conditions highlights its potential as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).

See full target information GINS2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 237:4487-4503 PubMed36251015

2022

ATM- and ATR-induced primary ciliogenesis promotes cisplatin resistance in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ying Chao,Bu-Miin Huang,I-Chen Peng,Pei-Rong Lee,Yi-Shyun Lai,Wen-Tai Chiu,Yi-Syuan Lin,Shih-Chieh Lin,Jung-Hsuan Chang,Pai-Sheng Chen,Shaw-Jenq Tsai,Chia-Yih Wang

Computational and mathematical methods in medicine 2022:1986348 PubMed36092790

2022

GINS2 Is Downregulated in Peripheral Blood of Patients with Intervertebral Disk Degeneration and Promotes Proliferation and Migration of Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Jiang,Hailang Sun,Jian Dai,Cheng Zhang,Xiaoming Tang

Molecular medicine reports 25: PubMed35137928

2022

GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Hu,Lina Ye,Zhe Liu

Journal of Cancer 13:610-622 PubMed35069907

2022

Identification of GINS2 prognostic potential and involvement in immune cell infiltration in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zuyin Li,Guohe Song,Dezhen Guo,Zhijie Zhou,Chen Qiu,Chao Xiao,Xiaoliang Wang,Yupeng Wang

Bioengineered 12:3336-3347 PubMed34288816

2021

MicroRNA miR-502-5p inhibits ovarian cancer genesis by downregulation of GINS complex subunit 2.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhan,Jing Yang,Yang Liu,Yanxiang Cheng,Hua Liu

OncoTargets and therapy 14:1219-1237 PubMed33658791

2021

TRPM2-AS Promotes Bladder Cancer by Targeting miR-22-3p and Regulating GINS2 mRNA Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yudong Tian,Yanbin Guan,Yang Su,Tao Yang,Haizhou Yu

Molecular medicine reports 23: PubMed33537829

2021

GINS2 affects cell proliferation, apoptosis, migration and invasion in thyroid cancer via regulating MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Saifei He,Miao Zhang,Ying Ye,Yanan Song,Xing Ma,Guoyu Wang,Juhua Zhuang,Wei Xia,Bin Zhao

OncoTargets and therapy 13:11433-11444 PubMed33192076

2020

Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqin Bu,Xiaojian Zhu,Xuan Yi,Chen Luo,Kang Lin,Jinfeng Zhu,Cegui Hu,Zitao Liu,Jiefeng Zhao,Chao Huang,Wenjun Zhang,Jun Huang

Cancer gene therapy : PubMed32747685

2020

GINS2 promotes EMT in pancreatic cancer via specifically stimulating ERK/MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Longping Huang,Si Chen,Haijun Fan,Dawei Ji,Chuanping Chen,Weiwei Sheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GINS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GINS2 antibody (AB197123)

Western blot - Anti-GINS2 antibody (AB197123)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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Product promise