AB25973

Anti-GIP antibody

Name: Anti-GIP antibody (ab25973) | Abcam
Brand: Abcam Limited
SKU: AB25973
Price: 510 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal GIP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human C1QTNF1.

View Alternative Names

CTRP1, UNQ310/PRO353, C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody (AB25973)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody (AB25973)

ab25973 at 10μg/ml staining GIP in human kidney tissue by IHC

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Western blot - Anti-GIP antibody (AB25973)

Lane 1:

Western blot - Anti-GIP antibody (ab25973) at 1 µg/mL

Lane 2:

Western blot - Anti-GIP antibody (ab25973) at 2 µg/mL

All lanes:

Human kidneycell lysate

Predicted band size: 32 kDa

Observed band size: 30 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-GIP antibody (AB25973)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GIP antibody (AB25973)

Immunofluorescence of CTRP1 in Human Kidney cells using ab25973 at 20 ug/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human C1QTNF1. The exact immunogen used to generate this antibody is proprietary information.

Q9BXJ1

Specificity

ab25973 recognises GIP (G protein coupled receptor interacting protein 1, GIP).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose-dependent Insulinotropic Polypeptide (GIP) also known as gastric inhibitory polypeptide is a hormone with a molecular mass around 5 kDa. It is a 42-amino acid peptide secreted by the K cells in the duodenum and jejunum of the small intestine. This protein interacts with the GIP receptor which is found in various tissues including pancreatic beta cells adipose tissue and the central nervous system. Researchers utilize GIP ELISA for detecting and quantifying this peptide in research studies.

Biological function summary

The GIP plays a significant role in stimulating insulin secretion in response to glucose intake which does not function alone. It works as part of the enteroinsular axis influencing nutrient uptake and metabolism. GIP also regulates fat metabolism by acting on adipocytes therefore playing a part in energy homeostasis. The exploration of GIP activity often involves MSD assay development to better understand its biological implications.

Pathways

The glucose-stimulated insulin secretion pathway incorporates GIP to enhance the release of insulin from the pancreas. It interacts with other proteins such as insulin and glucagon-like peptide-1 (GLP-1) to exert its glucose-lowering effects. GIP's involvement in the incretin pathway supports the body's maintenance of energy balance and glucose homeostasis under normal physiological conditions.

Glucose homeostasis dysfunction links GIP to type 2 diabetes and obesity. Impairments in GIP secretion or receptor signaling are associated with these metabolic disorders. Studies suggest that dysregulation of GIP along with related proteins like insulin can contribute to disease progression. By targeting GIP researchers at places like Abcam are looking for potential therapies to tackle these health challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information C1QTNF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 15:1357730 PubMed38595641

2024

Expression of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide in the rat submandibular gland is influenced by pre- and post-natal high-fat diet exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Pornchanok Sangsuriyothai,Ippei Watari,Saranya Serirukchutarungsee,Sirichom Satrawaha,Katarzyna Anna Podyma-Inoue,Takashi Ono

Biomolecules & biomedicine 24:939-951 PubMed38498315

2024

Dynamic network biomarker C1QTNF1 regulates tumor formation at the tipping point of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyuan Yu,Xijing Yan,Guanxing Chen,Rong Li,Zhou Yang,Zhixing Liang,Linsen Ye,Yunhao Chen,Yang Li

International journal of molecular sciences 24: PubMed37047812

2023

C1q/TNF-Related Proteins 1, 6 and 8 Are Involved in Corneal Epithelial Wound Closure by Targeting Relaxin Receptor RXFP1 In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hagen Fabian Nicolaus,Thomas Klonisch,Friedrich Paulsen,Fabian Garreis

International journal of molecular medicine 48: PubMed34278465

2021

Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Juan Chen,Yuan-Yuan Li,Ling-Ling Xia,Yong-Gui Wu

View all publications

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Product promise

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