Anti-GIP antibody [EPR20410] - BSA and Azide free

13 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Cytoplasmic staining on alpha cells of human pancreas islet is observed (PMID: 4574902).

  Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

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  Immunohistochemistry (Frozen sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

  Cytoplasmic staining on alpha cells of mouse pancreas islet is observed.

  The nuclear counterstain is DAPI (blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Sporadic and cytoplasmic staining on human duodenum is observed (PMID: 4574902).

  Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Cytoplasmic staining on alpha cells of mouse pancreas islet is observed (PMID: 4574902).

  Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Sporadic and cytoplasmic staining on mouse small intestine is observed (PMID: 4574902).

  Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GIP with Anti-GIP antibody [EPR20410] ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Cytoplasmic staining on alpha cells of rat pancreas islet is observed (PMID: 4574902).

  Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GIP antibody [EPR20410] ab209792).

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas labelling Somatostatin 28 with Anti-Somatostatin 28 antibody [RM1087] ab315106 at 1/7500 dilution (0.069 μg/ml) (B), GIP with ab271989 at 1/4000 dilution (0.27 μg/ml) (C) and Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain.

  Panel A: merged staining of anti-Carboxypeptidase A (gray; Opal™690), anti-GIP (green; Opal™520) and anti-Somatostatin 28 (red; Opal™570) on human pancreas.

  Panel B: anti-Somatostatin 28 stained on delta cells.

  Panel C: anti-GIP stained on alpha cells.

  Panel D: anti-Carboxypeptidase A stained on acinar cells.

  The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, ab271989, and Anti-Somatostatin 28 antibody [RM1087] ab315106 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-Carboxypeptidase A (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, gray; Opal™690), anti-GIP (ab271989, green; Opal™520) and anti-Somatostatin 28 (Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912, red; Opal™570) on human pancreas. Panel B: anti-Carboxypeptidase A stained on acinar cells. Panel C: anti-GIP stained on alpha cells. Panel D: anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml), ab271989 at 1/4000 dilution (0.27 μg/ml) and Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).

  Panel A: merged staining of anti-SV2A (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
  
  Panel B: anti-SV2A staining predominantly the beta cells in mouse pancreas islet.
  
  Panel C: anti-GIP staining the alpha cells in rat pancreas islet.
  
  Panel D: anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.

  Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of Anti-SV2A antibody [EPR23500-32] ab254351 at 1/1000 dilution (0.48 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Fluorescence multiplex immunohistochemical analysis of the mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections).

  Panel A: merged staining of anti-SV2A (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on mouse pancreas.
  
  Panel B: anti-SV2A staining predominantly the beta cells in mouse pancreas islet.
  
  Panel C: anti-GIP staining the alpha cells in mouse pancreas islet.
  
  Panel D: anti-Pancreatic Polypeptide staining the PP cells in mouse pancreas islet.

  Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of Anti-SV2A antibody [EPR23500-32] ab254351 at 1/1000 dilution (0.48 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Fluorescence multiplex immunohistochemical analysis of the mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections).

  Panel A: merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on mouse pancreas.
  
  Panel B: anti-Somatostatin 28 staining the delta cells in mouse pancreas islet.
  
  Panel C: anti-GIP staining the alpha cells in mouse pancreas islet.
  
  Panel D: anti-Pancreatic Polypeptide staining the PP cells in mouse pancreas islet.

  Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).

  Panel A: merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
  
  Panel B: anti-Somatostatin 28 staining the delta cells in rat pancreas islet.
  
  Panel C: anti-GIP staining the alpha cells in rat pancreas islet.
  
  Panel D: anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.

  Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining: in the order of Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and Anti-Pancreatic Polypeptide antibody [EPR22853-61] ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] - BSA and Azide free (ab271989)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human pancreas tissue staining Arx with Anti-Arx antibody [EPR27481-32] ab308260 at a 1/50 dilution ( 10.35 μg/ml) and ab271989 anti-GIP used at a 1/4000 dilution (0.27 μg/ml).
  
  Panel A: merged staining of anti-GIP (green; Opal™520) and anti-Homeobox protein ARX (red; Opal™570) on human pancreas.
  Panel B: anti-Homeobox protein ARX stained on alpha cells.
  Panel C: anti-GIP stained on alpha cells.
  
  The section was incubated in two rounds of staining: in the order of Anti-Arx antibody [EPR27481-32] ab308260 and ab271989 for 30 mins at room temperature.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.