AB134807

Anti-GIRK-3 antibody

Name: Anti-GIRK-3 antibody (ab134807) | Abcam
Brand: Abcam Limited
SKU: AB134807
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GIRK-3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KCNJ9.

View Alternative Names

GIRK3, KCNJ9, G protein-activated inward rectifier potassium channel 3, GIRK-3, Inward rectifier K(+) channel Kir3.3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (AB134807)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (AB134807)

Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Brain, Cortex Neurons tissue labelling GIRK-3 with ab134807 at 5 μg/ml.

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Western blot - Anti-GIRK-3 antibody (AB134807)

All lanes:

Western blot - Anti-GIRK-3 antibody (ab134807) at 1/500 dilution

Lane 1:

Protein extract from Lovo cells

Lane 2:

Protein extract from Lovo cells, treated with the synthesized peptide

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human KCNJ9. The exact immunogen used to generate this antibody is proprietary information.

Q92806

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein G-protein gated inwardly rectifying potassium channel 3 often abbreviated as GIRK-3 or Kir3.3 is part of the GIRK channel family. GIRK-3 transports potassium ions across cell membranes which contributes to maintaining the resting membrane potential and modulating cellular excitability. The molecular mass of GIRK-3 is approximately 43 kDa. GIRK-3 expresses properly in various tissues including the brain and heart where its activity regulates neuronal signaling and cardiac rhythm.

Biological function summary

The GIRK-3 protein functions as a subunit in a tetrameric complex with other GIRK channel family members such as GIRK-2 and GIRK-1. This complex formation is critical for the proper functioning of the channels allowing the selective conductance of potassium ions in response to G-protein signaling. The modulatory role of GIRK-3 in neuronal excitability and synaptic transmission is significant affecting neurotransmitter release and synaptic plasticity.

Pathways

GIRK-3 involvement is important in the dopaminergic and serotonergic signaling pathways. The protein impacts the transduction of signals mediated by G-protein coupled receptors (GPCRs) such as the dopamine D2 and serotonin 5-HT1A receptors. GIRK-3 interactions with GPCRs influence intracellular signaling cascades altering cellular responses to neurotransmitters. It works closely with the other GIRK family proteins and participates actively in signal modulation.

GIRK-3 exhibits connections to neurological disorders such as epilepsy and potentially to cardiac arrhythmias. The dysfunction of GIRK-3 along with its partner GIRK-2 in channel activity can lead to aberrant neuronal signaling contributing to seizure activity and abnormal heart rhythms. These associations highlight the importance of GIRK-3 in maintaining cellular homeostasis and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium (By similarity).

See full target information KCNJ9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1106434 PubMed37081973

2023

Tumor immune microenvironment of cutaneous angiosarcoma with cancer testis antigens and the formation of tertiary lymphoid structures.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Magara,Motoki Nakamura,Yuka Nojiri,Maki Yoshimitsu,Shinji Kano,Hiroshi Kato,Akimichi Morita

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GIRK-3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (AB134807)

Western blot - Anti-GIRK-3 antibody (AB134807)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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