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AB96168

Anti-GIRK1 antibody

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(1 Publication)

Rabbit Polyclonal GIRK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KCNJ3 aa 200 to C-terminus.

View Alternative Names

GIRK1, KCNJ3, G protein-activated inward rectifier potassium channel 1, GIRK-1, Inward rectifier K(+) channel Kir3.1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody (AB96168)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody (AB96168)

Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using ab96168 at 1 : 100 dilution.

Western blot - Anti-GIRK1 antibody (AB96168)
  • WB

Supplier Data

Western blot - Anti-GIRK1 antibody (AB96168)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GIRK1 antibody (ab96168) at 1/1000 dilution

Lane 1:

SH-SY5Y whole cell extracts at 30 µg

Lane 2:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KCNJ3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48549

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GIRK1 also known as Kir3.1 or KCNJ3 is a potassium channel protein that contributes to the regulation of potassium ion flow across cell membranes. It has a molecular mass of approximately 56 kDa. GIRK1 forms an important part of the inwardly rectifying potassium channels (Kir) and is expressed in various tissues most notably in the brain and heart. These channels allow the inward flow of K+ ions which are essential for many cellular processes.
Biological function summary

GIRK1 plays an important role in maintaining the resting membrane potential and neuronal excitability. It often forms a functional complex with other GIRK subunits such as GIRK2 and GIRK4 to mediate its effects. By responding to different neurotransmitters and coupling with G-protein-coupled receptors GIRK1 helps in neurotransmission regulation and contributes to the heart rate control.

Pathways

GIRK1 is involved in the G-protein coupled receptor (GPCR) signaling pathway and the atrial muscarinic acetylcholine receptor signaling pathway. This protein works closely with G-protein beta-gamma subunits which activate these pathways leading to increased potassium permeability. This regulation influences vital physiological processes like slowing the heart rate and modulating neural signals.

GIRK1 has connections to cardiac arrhythmias and epilepsy. Dysfunction in GIRK1-mediated channels can result in abnormal heart rhythms often linked with other proteins like GIRK4. In the nervous system aberrant GIRK1 function contributes to epilepsy by disrupting proper neuronal firing sometimes in association with altered G-protein signaling components. These associations highlight GIRK1 as a valuable target for therapeutic interventions in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins (PubMed : 8804710, PubMed : 8868049). This receptor plays a crucial role in regulating the heartbeat (By similarity).
See full target information KCNJ3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 4:e10150 PubMed26523391

2015

ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation.

Applications

Unspecified application

Species

Unspecified reactive species

Nataliya Nady,Ankit Gupta,Ziyang Ma,Tomek Swigut,Akiko Koide,Shohei Koide,Joanna Wysocka
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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