Anti-GIRK1 antibody [EPR29281-68]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in rat cerebellum (PMID : ©8604043). The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in mouse skeletal muscle. The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebellum. The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in rat skeletal muscle (PMID : ©8604043). The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebral cortex. The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling GIRK1 with ab320743 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in rat cerebral cortex. The section was incubated with ab320743 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

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Western blot - Anti-GIRK1 antibody [EPR29281-68] (AB320743)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, pancreas, skeletal muscle (PMID : 8604043).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30136838, 8604043).

Samples are non-boiled as boiling may cause protein aggregation.

Lanes 1-16 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lanes 17-20 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

The lanes 17-20 were developed using a high sensitivity ECL substrate.

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 180 seconds, lanes 2-16 : 37 seconds, lanes 17-20 : 59 seconds

All lanes:

Western blot - Anti-GIRK1 antibody [EPR29281-68] (ab320743) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 4:

Mouse hippocampus tissue lysate at 20 µg

Lane 5:

Mouse cerebellum tissue lysate at 20 µg

Lane 6:

Mouse olfactory bb tissue lysate at 20 µg

Lane 7:

Mouse spleen tissue lysate at 20 µg

Lane 8:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 9:

Mouse pancreas tissue lysate at 20 µg

Lane 10:

Rat hippocampus tissue lysate at 20 µg

Lane 11:

Rat cerebral cortex tissue lysate at 20 µg

Lane 12:

Rat brain tissue lysate at 20 µg

Lane 13:

Rat cerebellum tissue lysate at 20 µg

Lane 14:

Rat olfactory bulb tissue lysate at 20 µg

Lane 15:

Rat skeletal muscle tissue lysate at 20 µg

Lane 16:

Rat spleen tissue lysate at 20 µg

Lane 17:

human hippocampus tissue lysate at 20 µg

Lane 18:

Human cerebellum tissue lysate at 20 µg

Lane 19:

Human spleen tissue lysate at 20 µg

Lane 20:

Human skeletal muscle tissue lysate at 20 µg

Secondary

Lanes 1 - 16:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 17 - 20:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-75 kDa,36 kDa

false