AB65096

Anti-GIRK2 antibody

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(1 Review)

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(13 Publications)

Goat Polyclonal GIRK2 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-FrFl and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human KCNJ6 aa 350-400.

View Alternative Names

GIRK2, KATP2, KCNJ7, KCNJ6, G protein-activated inward rectifier potassium channel 2, GIRK-2, BIR1, Inward rectifier K(+) channel Kir3.2, KATP-2

1 Images

Western blot - Anti-GIRK2 antibody (AB65096)

WB

Supplier Data

Western blot - Anti-GIRK2 antibody (AB65096)

Primary incubation for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-GIRK2 antibody (ab65096) at 0.5 µg/mL

All lanes:

Human Brain (Substantia Nigra) tissue lysate in RIPA buffer at 35 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ELISA, IHC-FrFl, ICC/IF, IHC-P, WB

Immunogen

Synthetic Peptide within Human KCNJ6 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GIRK2 also known as G protein-coupled inwardly-rectifying potassium channel 2 or KCNJ6 serves as an inward rectifier potassium channel. Mechanically GIRK2 regulates the flow of K+ ions into the cell when activated by G proteins influencing cellular excitability. GIRK2 has a molecular weight of approximately 48 kDa. This protein is expressed in various tissues including the brain heart and pancreas where it plays a role in modulating neuronal signaling and cardiac rhythm.

Biological function summary

The GIRK2 channel contributes to maintaining resting membrane potentials and controlling neuronal excitability. GIRK2 forms heteromultimeric complexes with other GIRK subunits such as GIRK1 to fully function. These complexes are essential for the proper regulation of synaptic transmission and responsiveness to neurotransmitters particularly in areas of the brain like the cerebellum and hippocampus which are critical for cognitive and motor functions.

Pathways

GIRK2 plays a significant role in the GABAergic neurotransmission and dopaminergic pathways. In the GABAergic pathway GIRK2 aids in mediating inhibitory signals by enhancing the hyperpolarization of neurons therefore dampening neurotransmission. In dopaminergic pathways GIRK2 interacts closely with dopamine receptors to modulate dopamine-induced potassium conductance impacting processes related to mood and reward. These interactions implicate GIRK2 in the regulation of important signaling pathways related to neuronal function and mood regulation.

GIRK2 has been implicated in neurological conditions like Parkinson's disease and epilepsy. Alterations in GIRK2 expression or function can disrupt normal neuronal signaling potentially contributing to the pathophysiology of these disorders. In Parkinson's disease the dopaminergic neurons involving GIRK2 and its interactions with dopamine receptors become affected leading to motor dysfunction. Similarly in epilepsy abnormal GIRK2 function may impair synaptic inhibition resulting in uncontrolled neuronal excitability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors.

See full target information KCNJ6

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009388

2025

Neuroprotective Effects of Low-Dose Graphenic Materials on SN4741 Embryonic Stem Cells Against ER Stress and MPTP-Induced Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

David Vallejo Perez,Monica Navarro,Beatriz Segura-Segura,Rune Wendelbo,Sara Bandrés-Ciga,Miguel A Arraez,Cinta Arraez,Noela Rodriguez-Losada

iScience 28:113162 PubMed40799390

2025

Distinct microRNA signatures define sporadic PSP-RS and PD in patient-derived midbrain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Desirèe Valente,Clara Zannino,Stefania Scalise,Davide Bressan,Valeria Lucchino,Ermes Filomena,Giorgia Lucia Benedetto,Ida Lazzinnaro,Mariagrazia Talarico,Andrea Quattrone,Fulvio Chiacchiera,Graziano Pesole,Aldo Quattrone,Elvira Immacolata Parrotta,Giovanni Cuda

Development (Cambridge, England) 149: PubMed36305490

2022

Single-cell transcriptional and functional analysis of dopaminergic neurons in organoid-like cultures derived from human fetal midbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Marcella Birtele,Petter Storm,Yogita Sharma,Janko Kajtez,Jenny Nelander Wahlestedt,Edoardo Sozzi,Fredrik Nilsson,Simon Stott,Xiaoling L He,Bengt Mattsson,Daniella Rylander Ottosson,Roger A Barker,Alessandro Fiorenzano,Malin Parmar

Frontiers in cell and developmental biology 9:708389 PubMed34409038

2021

Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Gilmozzi,Giovanna Gentile,Diana A Riekschnitz,Michael Von Troyer,Alexandros A Lavdas,Emanuela Kerschbamer,Christian X Weichenberger,Marcelo D Rosato-Siri,Simona Casarosa,Luciano Conti,Peter P Pramstaller,Andrew A Hicks,Irene Pichler,Alessandra Zanon

Cell stem cell 28:112-126.e6 PubMed32966778

2020

Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function.

Applications

Unspecified application

Species

Unspecified reactive species

Man Xiong,Yezheng Tao,Qinqin Gao,Ban Feng,Wei Yan,Yingying Zhou,Thomas A Kotsonis,Tingli Yuan,Zhiwen You,Ziyan Wu,Jiajie Xi,Alexander Haberman,Julia Graham,Jasper Block,Wenhao Zhou,Yuejun Chen,Su-Chun Zhang

Cell 181:590-603.e16 PubMed32272060

2020

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Zhou,Jinlin Su,Xinde Hu,Changyang Zhou,He Li,Zhaorong Chen,Qingquan Xiao,Bo Wang,Wenyan Wu,Yidi Sun,Yingsi Zhou,Cheng Tang,Fei Liu,Linhan Wang,Canbin Feng,Mingzhe Liu,Sanlan Li,Yifeng Zhang,Huatai Xu,Haishan Yao,Linyu Shi,Hui Yang

Development (Cambridge, England) 146: PubMed31371375

2019

Laminin α2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development.

Applications

Unspecified application

Species

Unspecified reactive species

Maqsood Ahmed,Leandro N Marziali,Ernest Arenas,M Laura Feltri,Charles Ffrench-Constant

Stem cells international 2019:2945435 PubMed31236114

2019

Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Chabrat,Emmanuelle Lacassagne,Rodolphe Billiras,Sophie Landron,Amélie Pontisso-Mahout,Hélène Darville,Alain Dupront,Francis Coge,Esther Schenker,David Piwnica,Emmanuel Nivet,François Féron,Clotilde Mannoury la Cour

Stem cell reports 12:518-531 PubMed30799274

2019

Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwon Kim,Hyeok Ju Park,Hwan Choi,Yujung Chang,Hanseul Park,Jaein Shin,Junyeop Kim,Christopher J Lengner,Yong Kyu Lee,Jongpil Kim

Cell stem cell 23:123-131.e6 PubMed29979986

2018

Th17 Lymphocytes Induce Neuronal Cell Death in a Human iPSC-Based Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Sommer,Franz Marxreiter,Florian Krach,Tanja Fadler,Janina Grosch,Michele Maroni,Daniela Graef,Esther Eberhardt,Markus J Riemenschneider,Gene W Yeo,Zacharias Kohl,Wei Xiang,Fred H Gage,Jürgen Winkler,Iryna Prots,Beate Winner

View all publications

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