Goat Polyclonal GIRK2 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-FrFl and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human KCNJ6 aa 350-400.
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Publications
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- Development (Cambridge, England) 149:2022Single-cell transcriptional and functional analysis of dopaminergic neurons in organoid-like cultures derived from human fetal midbrain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcella Birtele,Petter Storm,Yogita Sharma,Janko Kajtez,Jenny Nelander Wahlestedt,Edoardo Sozzi,Fredrik Nilsson,Simon Stott,Xiaoling L He,Bengt Mattsson,Daniella Rylander Ottosson,Roger A Barker,Alessandro Fiorenzano,Malin ParmarPubMed 36305490
- Frontiers in cell and developmental biology 9:7083892021Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Gilmozzi,Giovanna Gentile,Diana A Riekschnitz,Michael Von Troyer,Alexandros A Lavdas,Emanuela Kerschbamer,Christian X Weichenberger,Marcelo D Rosato-Siri,Simona Casarosa,Luciano Conti,Peter P Pramstaller,Andrew A Hicks,Irene Pichler,Alessandra ZanonPubMed 34409038
- Cell stem cell 28:112-126.e62020Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Xiong,Yezheng Tao,Qinqin Gao,Ban Feng,Wei Yan,Yingying Zhou,Thomas A Kotsonis,Tingli Yuan,Zhiwen You,Ziyan Wu,Jiajie Xi,Alexander Haberman,Julia Graham,Jasper Block,Wenhao Zhou,Yuejun Chen,Su-Chun ZhangPubMed 32966778
- Development (Cambridge, England) 146:2019Laminin α2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaqsood Ahmed et. al.PubMed 31371375
- Stem cells international 2019:29454352019Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAudrey Chabrat et. al.PubMed 31236114
- Stem cell reports 12:518-5312019Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwon Kim et. al.PubMed 30799274
- Cell stem cell 23:123-131.e62018Th17 Lymphocytes Induce Neuronal Cell Death in a Human iPSC-Based Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnika Sommer,Franz Marxreiter,Florian Krach,Tanja Fadler,Janina Grosch,Michele Maroni,Daniela Graef,Esther Eberhardt,Markus J Riemenschneider,Gene W Yeo,Zacharias Kohl,Wei Xiang,Fred H Gage,Jürgen Winkler,Iryna Prots,Beate WinnerPubMed 29979986
- The Journal of neuroscience : the official journal 38:1662-16762018BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons and in Human Induced Pluripotent and Neural Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVukasin M Jovanovic et. al.PubMed 29321139
- Frontiers in cellular neuroscience 10:2912016Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells.
- PloS one 9:e873882014Physiological characterisation of human iPS-derived dopaminergic neurons.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KCNJ6 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48051
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | IHC-FrFl | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Expected |
| Mouse | Expected | Predicted | Expected | Expected | Expected |
| Rat | Expected | Predicted | Expected | Expected | Expected |
| Orangutan | Predicted | Predicted | Predicted | Predicted | Predicted |
| Syrian hamster | Predicted | Predicted | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan, Syrian hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Orangutan, Syrian hamster, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan, Syrian hamster | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan, Syrian hamster | Dilution info - | Notes - |
IHC-FrFl
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan, Syrian hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Alternative names
GIRK2, KATP2, KCNJ7, KCNJ6, G protein-activated inward rectifier potassium channel 2, GIRK-2, BIR1, Inward rectifier K(+) channel Kir3.2, KATP-2
Recommended products
Goat Polyclonal GIRK2 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-FrFl and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human KCNJ6 aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KCNJ6 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48051
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GIRK2 also known as G protein-coupled inwardly-rectifying potassium channel 2 or KCNJ6 serves as an inward rectifier potassium channel. Mechanically GIRK2 regulates the flow of K+ ions into the cell when activated by G proteins influencing cellular excitability. GIRK2 has a molecular weight of approximately 48 kDa. This protein is expressed in various tissues including the brain heart and pancreas where it plays a role in modulating neuronal signaling and cardiac rhythm.
- Biological function summary
The GIRK2 channel contributes to maintaining resting membrane potentials and controlling neuronal excitability. GIRK2 forms heteromultimeric complexes with other GIRK subunits such as GIRK1 to fully function. These complexes are essential for the proper regulation of synaptic transmission and responsiveness to neurotransmitters particularly in areas of the brain like the cerebellum and hippocampus which are critical for cognitive and motor functions.
- Pathways
GIRK2 plays a significant role in the GABAergic neurotransmission and dopaminergic pathways. In the GABAergic pathway GIRK2 aids in mediating inhibitory signals by enhancing the hyperpolarization of neurons therefore dampening neurotransmission. In dopaminergic pathways GIRK2 interacts closely with dopamine receptors to modulate dopamine-induced potassium conductance impacting processes related to mood and reward. These interactions implicate GIRK2 in the regulation of important signaling pathways related to neuronal function and mood regulation.
- Associated diseases and disorders
GIRK2 has been implicated in neurological conditions like Parkinson's disease and epilepsy. Alterations in GIRK2 expression or function can disrupt normal neuronal signaling potentially contributing to the pathophysiology of these disorders. In Parkinson's disease the dopaminergic neurons involving GIRK2 and its interactions with dopamine receptors become affected leading to motor dysfunction. Similarly in epilepsy abnormal GIRK2 function may impair synaptic inhibition resulting in uncontrolled neuronal excitability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Western blot - Anti-GIRK2 antibody (ab65096)
Primary incubation for 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-GIRK2 antibody (ab65096) at 0.5 µg/mL
All lanes: Human Brain (Substantia Nigra) tissue lysate in RIPA buffer at 35 µg
Predicted band size: 48 kDa
Observed band size: 48 kDa
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