AB256311

Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GIRK2 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Girk2, Kcnj7, W, Kcnj6, G protein-activated inward rectifier potassium channel 2, GIRK-2, Inward rectifier K(+) channel Kir3.2

2 Images

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Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (AB256311)

GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1 : rat brain lysate (10μg) (Input).

Lane 2 : ab229913 IP in rat brain lysate (Input).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Exposure time : 7 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229913).

All lanes:

Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (<a href='/en-us/products/primary-antibodies/girk2-antibody-epr22347-2-ab229913'>ab229913</a>)

Predicted band size: 48 kDa

Observed band size: 48 kDa

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Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (AB256311)

GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1 : Mouse hippocampus lysate (10μg) (Input).

Lane 2 : ab229913 IP in mouse hippocampus lysate (Input).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 7 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229913).

All lanes:

Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (<a href='/en-us/products/primary-antibodies/girk2-antibody-epr22347-2-ab229913'>ab229913</a>)

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Unconjugated

Anti-GIRK2 antibody [EPR22347-2]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22347-2

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab256311 is the carrier-free version of ab229913.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GIRK2 also known as G protein-coupled inwardly-rectifying potassium channel 2 or KCNJ6 serves as an inward rectifier potassium channel. Mechanically GIRK2 regulates the flow of K+ ions into the cell when activated by G proteins influencing cellular excitability. GIRK2 has a molecular weight of approximately 48 kDa. This protein is expressed in various tissues including the brain heart and pancreas where it plays a role in modulating neuronal signaling and cardiac rhythm.

Biological function summary

The GIRK2 channel contributes to maintaining resting membrane potentials and controlling neuronal excitability. GIRK2 forms heteromultimeric complexes with other GIRK subunits such as GIRK1 to fully function. These complexes are essential for the proper regulation of synaptic transmission and responsiveness to neurotransmitters particularly in areas of the brain like the cerebellum and hippocampus which are critical for cognitive and motor functions.

Pathways

GIRK2 plays a significant role in the GABAergic neurotransmission and dopaminergic pathways. In the GABAergic pathway GIRK2 aids in mediating inhibitory signals by enhancing the hyperpolarization of neurons therefore dampening neurotransmission. In dopaminergic pathways GIRK2 interacts closely with dopamine receptors to modulate dopamine-induced potassium conductance impacting processes related to mood and reward. These interactions implicate GIRK2 in the regulation of important signaling pathways related to neuronal function and mood regulation.

GIRK2 has been implicated in neurological conditions like Parkinson's disease and epilepsy. Alterations in GIRK2 expression or function can disrupt normal neuronal signaling potentially contributing to the pathophysiology of these disorders. In Parkinson's disease the dopaminergic neurons involving GIRK2 and its interactions with dopamine receptors become affected leading to motor dysfunction. Similarly in epilepsy abnormal GIRK2 function may impair synaptic inhibition resulting in uncontrolled neuronal excitability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins (By similarity). Forms a functional channel in association with KCNJ3/GIRK1 (PubMed : 10341034).. Isoform GIRK2-1. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins.. Isoform GIRK2B. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins.. Isoform GIRK2D. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins.

See full target information Kcnj6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1013262 PubMed36684586

2023

SARS-CoV-2 Spike protein activates TMEM16F-mediated platelet procoagulant activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ambra Cappelletto,Harriet E Allan,Marilena Crescente,Edoardo Schneider,Rossana Bussani,Hashim Ali,Ilaria Secco,Simone Vodret,Roberto Simeone,Luca Mascaretti,Serena Zacchigna,Timothy D Warner,Mauro Giacca

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free

Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (AB256311)

Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (AB256311)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise