AB203297

Anti-GITR antibody

Name: Anti-GITR antibody (ab203297) | Abcam
Brand: Abcam Limited
SKU: AB203297
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GITR antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Tnfrsf18 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody (AB203297)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody (AB203297)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human oral squamous cell carcinoma tissue labeling GITR with ab203297 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody (AB203297)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse lymph node tissue labeling GITR with ab203297 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Tnfrsf18 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O35714

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.

Biological function summary

GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.

Pathways

The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.

GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

See full target information TNFRSF18

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:2 PubMed36596993

2023

Aβ efflux impairment and inflammation linked to cerebrovascular accumulation of amyloid-forming amylin secreted from pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Nirmal Verma,Gopal Viswanathan Velmurugan,Edric Winford,Han Coburn,Deepak Kotiya,Noah Leibold,Laura Radulescu,Sanda Despa,Kuey C Chen,Linda J Van Eldik,Peter T Nelson,Donna M Wilcock,Gregory A Jicha,Ann M Stowe,Larry B Goldstein,David K Powel,Jeffrey H Walton,Manuel F Navedo,Matthew A Nystoriak,Andrew J Murray,Geert Jan Biessels,Claire Troakes,Henrik Zetterberg,John Hardy,Tammaryn Lashley,Florin Despa

View all publications

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Product promise

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