AB264419

Anti-GITR antibody [BLR068G] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal GITR antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus.

View Alternative Names

CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein

5 Images

Supplier Data

Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

Immocytochemistry analysis of formalin-fixed, paraffin embedded human RPMI-8226 cells labeling GITR with ab264419 at a 1/125 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.

Supplier Data

Western blot - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

Lysates prepared using NETN buffer.

All lanes:

Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419) at 1/1000 dilution

Lane 1:

MJ whole cell lysate at 15 µg

Lane 2:

Jurkat whole cell lysate at 50 µg

Lane 3:

RMPI-8226 whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Lane 5:

Raji whole cell lysate at 50 µg

Lane 6:

MOLT4 whole cell lysate at 50 µg

Lane 7:

Daudi whole cell lysate at 50 µg

Lane 8:

C5/MJ whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 26 kDa

true

Exposure time: 30s

Supplier Data

Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

GITR was immunoprecipitated from MJ whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264419 at 6 μl per reaction. Western blot was performed from the immunoprecipitates using ab264419 at 1/1000.

Lane 1 : ab264419 IP in MJ whole cell lysate.
Lane 2 : Control IgG IP in MJ whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Predicted band size: 26 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/200 dilution. Secondary : DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain : DAPI (blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/125 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR068G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5U5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 6 μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.

Biological function summary

GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.

Pathways

The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.

GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

See full target information TNFRSF18

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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gitr-antibody-cal61-ab237725

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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