Rabbit Recombinant Monoclonal GITR antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus.
Images
![Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (AB264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunocytochemistry-img97509.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (AB264419)")
![Western blot - Anti-GITR antibody [BLR068G] - BSA free (AB264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--western-blot-img106406.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GITR antibody [BLR068G] - BSA free (AB264419)")
![Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (AB264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunoprecipitation-img69614.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (AB264419)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112205.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134837.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (AB264419)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5U5
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 6 μl/mg lysate. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Alternative names
AITR, GITR, UNQ319/PRO364, AITR, GITR, TNFRSF18, UNQ319/PRO364, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein
Recommended products
Rabbit Recombinant Monoclonal GITR antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human TNFRSF18 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5U5
- Clone number
BLR068G
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.
- Biological function summary
GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.
- Pathways
The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.
- Associated diseases and disorders
GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (ab264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunocytochemistry-img97509.jpg "Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (ab264419)")
Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
Immocytochemistry analysis of formalin-fixed, paraffin embedded human RPMI-8226 cells labeling GITR with ab264419 at a 1/125 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
![Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--western-blot-img106406.jpg "Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419)")
Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
Lysates prepared using NETN buffer.
All lanes: Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419) at 1/1000 dilution
Lane 1: MJ whole cell lysate at 15 µg
Lane 2: Jurkat whole cell lysate at 50 µg
Lane 3: RMPI-8226 whole cell lysate at 50 µg
Lane 4: HeLa whole cell lysate at 50 µg
Lane 5: Raji whole cell lysate at 50 µg
Lane 6: MOLT4 whole cell lysate at 50 µg
Lane 7: Daudi whole cell lysate at 50 µg
Lane 8: C5/MJ whole cell lysate at 15 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 30s
![Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunoprecipitation-img69614.jpg "Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419)")
Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
GITR was immunoprecipitated from MJ whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264419 at 6 μl per reaction. Western blot was performed from the immunoprecipitates using ab264419 at 1/1000.
Lane 1: ab264419 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112205.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/200 dilution. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-blr068g-bsa-free-ab264419--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134837.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/125 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com