Rabbit Recombinant Monoclonal GITR antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131629.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)")
![Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunoprecipitation-img3986.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110976.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)")
![Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunoprecipitation-img138640.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)")
![Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--flow-cytometry-intracellular-img119264.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Boil tissue section in TRIS EDTA buffer for 24 min followed by cooling at room temperature for 30-45 min. Primary antibody incubation for 75 minutes at room temperature. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Alternative names
Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein, AITR, GITR, TNFRSF18, UNQ319/PRO364
Recommended products
Rabbit Recombinant Monoclonal GITR antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
CAL52
- Purification technique
Affinity purification Protein A
- Concentration
- Purification notes
Purity is greater than 99%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251600 is the carrier-free version of Anti-GITR antibody [CAL52] ab237713.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.
- Biological function summary
GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.
- Pathways
The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.
- Associated diseases and disorders
GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131629.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with Anti-GITR antibody [CAL52] ab237713 at 1/4000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human tonsil is observed. Counter stained with Hematoxylin. The section was incubated with Anti-GITR antibody [CAL52] ab237713 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
![Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunoprecipitation-img3986.jpg "Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
GITR was immunoprecipitated from 0.35 mg Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate using Anti-GITR antibody [CAL52] ab237713 at 1/30 dilution. western blot was performed on the immunoprecipitate using Anti-GITR antibody [CAL52] ab237713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Hut-78 whole cell lysate 10 μg (input)
Lane 2: Anti-GITR antibody [CAL52] ab237713 IP in Hut-78 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GITR antibody [CAL52] ab237713 in Hut-78 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
This blot was developed using a higher sensitivity ECL substrate.
Dimerized GITR was also observed at 52kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
All lanes: Immunoprecipitation - Anti-GITR antibody [CAL52] (Anti-GITR antibody [CAL52] ab237713)
Predicted band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110976.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for TNFSF18 using Anti-GITR antibody [CAL52] ab237713 at 0.25 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
![Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunoprecipitation-img138640.jpg "Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
GITR was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with GFP-tagged GITR overexpression vector whole cell lysate using Anti-GITR antibody [CAL52] ab237713 at 1/30 dilution. western blot was performed on the immunoprecipitate using Anti-GITR antibody [CAL52] ab237713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Hut-78 whole cell lysate 10 μg (input)
Lane 2: Anti-GITR antibody [CAL52] ab237713 IP in Hut-78 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GITR antibody [CAL52] ab237713 in 293T transfected with GFP-tagged GITR overexpression vector whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
Dimerized GITR was also observed at 52kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
All lanes: Immunoprecipitation - Anti-GITR antibody [CAL52] (Anti-GITR antibody [CAL52] ab237713)
Predicted band size: 26 kDa
![Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--flow-cytometry-intracellular-img119264.jpg "Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 48h, labeling GITRwith Anti-GITR antibody [CAL52] ab237713 at 1/500 dilution. The secondary antibody was a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/500 dilution. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellular staining rabbit IgG (Left) or Anti-GITR antibody [CAL52] ab237713 (Right). The isotype control used was a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Left).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
![Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunocytochemistry-immunofluorescence-img45133.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling GITR with Anti-GITR antibody [CAL52] ab237713 at 1/50 dilution followed by a AlexaFluor®594 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at a 1/500 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing Positive staining in HEK-293T cells transfected with a GFP-tagged GITR expression construct.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®594 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at a 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GITR antibody [CAL52] ab237713).
![Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunocytochemistry-immunofluorescence-img45133.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)")
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