Rabbit Recombinant Monoclonal GITR antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66724.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11974.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725)")
![Western blot - Anti-GITR antibody [CAL61] (AB237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--western-blot-img11057.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GITR antibody [CAL61] (AB237725)")
![Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL61] (AB237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--flow-cytometry-intracellular-img119265.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL61] (AB237725)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (AB237725)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Not recommended | Tested | Not recommended | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway (By similarity).
Alternative names
Gitr, Tnfrsf18, Gitr, Tumor necrosis factor receptor superfamily member 18, Glucocorticoid-induced TNFR-related protein
Recommended products
Rabbit Recombinant Monoclonal GITR antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
CAL61
- Purification technique
Affinity purification Protein A
- Concentration
- Purification notes
Purity >99%
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.
- Biological function summary
GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.
- Pathways
The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.
- Associated diseases and disorders
GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66724.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse lung is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11974.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-GITR antibody [CAL61] (ab237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--western-blot-img11057.jpg "Western blot - Anti-GITR antibody [CAL61] (ab237725)")
Western blot - Anti-GITR antibody [CAL61] (ab237725)
33 kDa band represents GITR isoform B based on Uniprot and literature. (PMID: 10836847).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GITR antibody [CAL61] (ab237725) at 1/1000 dilution
Lane 1: Untreated mouse splenocytes whole cell lysate at 20 µg
Lane 2: Mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Exposure time: 3min
![Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL61] (ab237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--flow-cytometry-intracellular-img119265.jpg "Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL61] (ab237725)")
Flow Cytometry (Intracellular) - Anti-GITR antibody [CAL61] (ab237725)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed,0.1% Tween-20permeabilized mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days labeling GITR with ab237725 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab237725 (Right).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725), expandable thumbnail](https://content.abcam.com/products/images/gitr-antibody-cal61-ab237725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)
Formalin-fixed, paraffin-embedded mouse spleen tissue stained for GITR using ab237725 at 0.125 μg/ml dilution in immunohistochemical analysis.
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