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AB68807

Anti-GIYD2 antibody

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(1 Publication)

Mouse Polyclonal GIYD2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SLX1A.

View Alternative Names

GIYD1, SLX1, SLX1B, GIYD2, SLX1A, Structure-specific endonuclease subunit SLX1, GIY-YIG domain-containing protein 1

1 Images
Western blot - Anti-GIYD2 antibody (AB68807)
  • WB

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Western blot - Anti-GIYD2 antibody (AB68807)

All lanes:

Western blot - Anti-GIYD2 antibody (ab68807) at 1/500 dilution

Lane 1:

GIYD2 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SLX1A.

Q9BQ83

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GIYD2 also known as GID complex subunit 2 is a protein with a molecular mass of approximately 36 kDa. This protein belongs to a family of proteins that contain the GYF domain involved in protein-protein interactions. GIYD2 shows expression in various tissues with significant presence in the brain liver and kidneys. The protein plays a role in maintaining cellular processes by potentially interacting with other proteins through its GYF domain.
Biological function summary

The GIYD2 protein functions within the GID complex which participates in the degradation of specific proteins via the ubiquitin-proteasome system. This process involves tagging target proteins with ubiquitin molecules directing them to the proteasome for degradation. The activity of GIYD2 within the complex ensures that levels of particular proteins are kept in check facilitating normal cellular functions and responses. The proper function of GIYD2 helps prevent the accumulation of dysfunctional or unnecessary proteins in the cell.

Pathways

GIYD2 interacts in the ubiquitin-mediated proteolysis pathway influencing protein turnover in the cell. Its action is related to the action of ubiquitin ligases such as UBR1 which assists in recognizing proteins marked for degradation. GIYD2 also impacts the cell cycle pathway by regulating the stability of specific cell cycle regulators linking it to proteins involved in cell division and growth such as cyclins and cyclin-dependent kinases.

GIYD2 has connections to neurodegeneration and liver diseases where protein homeostasis is disrupted. Abnormal GIYD2 function may impact levels of proteins influencing neurodegenerative disorders like Alzheimer's disease in association with proteins such as tau. In liver diseases its involvement in protein degradation may affect responses to cellular stress and damage interacting with cellular stress response elements including heat shock proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products.
See full target information SLX1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:1290-1314 PubMed39870965

2025

The BLM-TOP3A-RMI1-RMI2 proximity map reveals that RAD54L2 suppresses sister chromatid exchanges.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Jennifer Ho,Edith Cheng,Cassandra J Wong,Jonathan R St-Germain,Wade H Dunham,Brian Raught,Anne-Claude Gingras,Grant W Brown
View all publications
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