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AB203564

Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker

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(1 Publication)

Rabbit Recombinant Monoclonal GJA3/Cx46 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Gja3 aa 100-150.

View Alternative Names

Gap junction alpha-3 protein, Connexin-46, Cx46, Gja3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker (AB203564)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker (AB203564)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse lens tissue labeling GJA3/Cx46 with ab203564 at 1 μg/mL.

Western blot - Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker (AB203564)
  • WB

Supplier Data

Western blot - Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker (AB203564)

All lanes:

Western blot - Anti-GJA3/Cx46 antibody [11H16L33] - Intercellular Junction Marker (ab203564) at 2 µg/mL

All lanes:

mouse lens lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

11H16L33

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Gja3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q64448

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GJA3 also known as Connexin 46 (Cx46) is a gap junction protein essential for cell communication. The protein has a molecular mass of about 46 kDa. It forms intercellular channels that connect adjacent cells allowing for the transfer of ions and small molecules. GJA3 is mostly expressed in the lens of the eye where it plays an important role in maintaining lens transparency and homeostasis.
Biological function summary

GJA3 participates in the formation of gap junctions that are important for maintaining cellular processes in the eye lens. As part of a connexin family complex GJA3 interacts with other connexin proteins like Cx50 to ensure proper cell-to-cell communication. This interaction is vital for the metabolic cooperation between lens fibers and helps in maintaining the avascular nature of the lens.

Pathways

GJA3 contributes to the regulation of lens fiber cell differentiation and growth pathways. It closely interacts with the and phosphoinositide 3-kinase (PI3K) and calcium signaling pathways. These pathways are critical for lens development and for responding to stress within the lens. In conjunction with Cx50 GJA3 modulates these signaling pathways to regulate lens opacity and health.

Mutations in GJA3 associate with congenital cataracts which lead to vision impairment or loss. These cataracts often result from disrupted intercellular communication within the lens affecting lens transparency. Additionally alterations in GJA3 impact the function of Cx50 another lens-specific connexin and can exacerbate the severity of cataract formation. Understanding the GJA3 and Cx50 interaction gives insights into the pathological mechanisms behind lens-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.
See full target information Gja3
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Publications (1)

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Cancer immunology, immunotherapy : CII 73:82 PubMed38554200

2024

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Applications

Unspecified application

Species

Unspecified reactive species

Zhe Ma,Zhenxing Zhou,Wenwen Duan,Gaofeng Yao,Shimei Sheng,Sidou Zong,Xin Zhang,Changkui Li,Yuanyuan Liu,Fengting Ou,Maha Raja Dahar,Yanshan Huang,Lushan Yu
View all publications
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Product promise

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