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Rabbit Recombinant Monoclonal Cx47 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (AB303507), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (AB303507), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (AB303507), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (AB303507), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (AB303507), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Tested
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Cx47 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26419-75
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GJC2 also known as Gap Junction Protein Gamma 2 or Connexin 47 (Cx47) acts in cell-to-cell communication through the formation of gap junction channels. This protein has a mass of about 47 kDa. It expresses largely in oligodendrocytes within the central nervous system as well as in Schwann cells in the peripheral nervous system. These junctions allow the transfer of ions and small molecules facilitating coordinated responses among cells important for nervous system function.

Biological function summary

GJC2 facilitates the transport of signaling molecules between neural cells. It integrates into gap junction complexes essential for maintaining the myelin structure in nerve cells providing proper electrical insulation. This protein's activity impacts the conduction velocity of nerve impulses. Its function in maintaining neural signaling underlines its key role in supporting the complex neural network facilitating proper cellular interactions and communication.

Pathways

GJC2 is critical in the neural signaling pathways related to myelin maintenance and gap junction organization. It associates closely with other connexins such as Connexin 32 (Cx32) within these pathways. The interaction among different connexins orchestrates cell signaling contributing to the homeostasis and electrical conductance in neural circuits essential for normal nervous system operations.

Associated diseases and disorders

GJC2 mutations link to neurological conditions such as Pelizaeus-Merzbacher-like disease (PMLD) and Spastic Paraplegia type 44. Each of these disorders involves dysfunctions in myelin formation and maintenance leading to spasticity and other motor deficits. GJC2-related diseases often correlate with malfunctions or compensatory changes in other connexin proteins which further contributes to clinical variability and progression of these myelin disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

    This data was developed using ab30506, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GJC2 with Anti-GJC2 antibody [EPR26419-75] ab303506 at 1/8000 (0.067 µg/ml) followed by a Leica DS9800 (Bond™ Polymer Refine Detection) kit at a ready to use dilution. Negative control: no staining on mouse kidney. The section was incubated with Anti-GJC2 antibody [EPR26419-75] ab303506 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a Leica DS9800 (Bond™ Polymer Refine Detection) kit used at a ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

    This data was developed using Anti-GJC2 antibody [EPR26419-75] ab303506, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GJC2 with Anti-GJC2 antibody [EPR26419-75] ab303506 at 1/50 (10.7 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GJC2 antibody [EPR26419-75] ab303506 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GJC2 antibody [EPR26419-75] - BSA and Azide free (ab303507)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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