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AB153926

Anti-GK2 antibody

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(1 Publication)

Rabbit Polyclonal GK2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GK2 aa 1-350.

View Alternative Names

GKP2, GKTA, GK2, Glycerol kinase 2, GK 2, Glycerokinase 2, ATP:glycerol 3-phosphotransferase 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (AB153926)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (AB153926)

Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue, labeling GK2 with ab153926 at 1/100 dilution.

Western blot - Anti-GK2 antibody (AB153926)
  • WB

Unknown

Western blot - Anti-GK2 antibody (AB153926)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GK2 antibody (ab153926) at 1/500 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GK2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q14410

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GK2 also known as Glycerol Kinase 2 is primarily an enzyme that catalyzes the phosphorylation of glycerol to form glycerol-3-phosphate. This enzyme activity is essential in lipid metabolism. The protein is relatively small with a molecular mass of about 60 kDa. Expression of GK2 occurs mostly in the testes although it is also found in other tissues indicating a specific but not exclusive role in male physiology.
Biological function summary

GK2 contributes significantly to the metabolic processes involving glycerol and energy storage. It functions as part of an enzymatic sequence for transforming glycerol into a form usable for energy production. While GK2 is not traditionally associated with complex structures it interacts closely with other enzymes in the glycerol-3-phosphate pathway to maintain its function. This interaction ensures effective regulation of glycerol levels in cells aiding metabolic balance.

Pathways

GK2 plays a significant role in lipid metabolism and energy homeostasis. The glycerol-3-phosphate shuttle utilizes GK2 to facilitate the transfer of reducing equivalents into mitochondria linking it to oxidative phosphorylation. Proteins such as glycerol-3-phosphate dehydrogenase are related to GK2 within these pathways providing a tight regulation and ensuring the efficient flow of metabolites through the cycle.

GK2 dysfunction appears connected to metabolic syndromes and fertility issues. Variations in GK2 expression and activity have been observed in cases of metabolic syndrome impacting overall lipid levels. In disorders related to male fertility GK2 interactions with proteins like glycerol-3-phosphate dehydrogenase could influence energy supply affecting spermatogenesis and fertility outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key enzyme in the regulation of glycerol uptake and metabolism. Essential for male fertility and sperm mitochondrial sheath formation (By similarity). Required for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during spermatogenesis (By similarity). Can induce mitochondrial clustering through interactions with PLD6 and up-regulation of phosphatidic acid synthesis in the mitochondria (PubMed : 28852571).
See full target information GK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38739438

2024

Retinal metabolism displays evidence for uncoupling of glycolysis and oxidative phosphorylation via Cori-, Cahill-, and mini-Krebs-cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyi Chen,Laimdota Zizmare,Victor Calbiague,Lan Wang,Shirley Yu,Fritz W Herberg,Oliver Schmachtenberg,Francois Paquet-Durand,Christoph Trautwein
View all publications
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Product promise

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