AB188866

Anti-GKN2 antibody [EPR15377(B)] - C-terminal

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal GKN2 antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 7 publications.

View Alternative Names

BLOT, GDDR, TFIZ1, UNQ465/PRO813, GKN2, Gastrokine-2, Blottin, Down-regulated in gastric cancer, Trefoil factor interactions(z) 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab188866 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Western blot analysis of GKN2 in Human stomach lysate immunoprecipitated using ab188866 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Predicted band size: 20 kDa

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GKN2 with ab188866 at 1/4,000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

All lanes:

Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866) at 1/5000 dilution

Lane 1:

Human stomach lysate at 20 µg

Lane 2:

Mouse stomach lysate at 20 µg

Lane 3:

Rat stomach lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Carrier free

Anti-GKN2 antibody [EPR15377(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15377(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gastrokine 2 commonly known as GKN2 is a protein involved in maintaining gastric mucosal integrity. It has an approximate molecular weight of 21 kDa. GKN2 is mainly expressed in the stomach lining particularly in gastric epithelial cells. Alternate names for GKN2 include BRICD1B and CA11ORF18. By contributing to the mucus layer GKN2 helps to protect the underlying tissues from digestive acids and enzymes.

Biological function summary

The role of GKN2 includes important activities within the gastric mucosa. It is known to form part of a protective complex in the stomach that maintains epithelial homeostasis. GKN2 interacts with other gastrokines like GKN1 to support mucosal repair and cell proliferation. It promotes gastric epithelial cell differentiation and inhibits apoptosis which helps control the integrity and function of the stomach lining.

Pathways

GKN2 becomes integral in gastric epithelial cell signaling and repair. It associates closely with pathways related to cell cycle regulation and inflammation control within the gastric environment. GKN1 a related protein often co-regulates these processes alongside GKN2. Their interaction supports gastric healing processes reducing unnecessary cell loss and improving mucosal resilience.

GKN2 shows involvement in gastric cancer and gastritis. Alterations in GKN2 expression can indicate a disruption in gastric mucosal defenses often observed in gastric cancer development. In gastroduodenal disorders like gastritis low levels of GKN2 can correlate with increased inflammation and epithelial damage. GKN1 also shows significant connection to these diseases by interacting with GKN2 to modulate protective mechanisms in the gastric environment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information GKN2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0332769 PubMed40997129

2025

Multimodal analysis investigating the shared pathogenic mechanisms of osteoporosis and osteoarthritis with an initial exploration of the role of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunqing Wang,Dongcheng Ran,Wenyi Li,Cheng Yang,Weimin Li

International journal of biological sciences 20:2323-2338 PubMed38617533

2024

IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1.

Applications

Unspecified application

Species

Unspecified reactive species

Kewei Liu,Hongxia Huang,Mengyuan Xiong,Qiaojiao Wang,Xiantao Chen,Yinqiong Feng,Hang Ma,Wanqun Chen,Xuegang Li,Xiaoli Ye

Science advances 9:eadf6210 PubMed37494452

2023

Phosphorylated nuclear DICER1 promotes open chromatin state and lineage plasticity of AT2 tumor cells in lung adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Raisa A Reyes-Castro,Shin-Yu Chen,Jacob Seemann,Samrat T Kundu,Don L Gibbons,Swathi Arur

Nature communications 14:3750 PubMed37386010

2023

Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Takada,Yohei Sasagawa,Mika Yoshimura,Kaori Tanaka,Yoshimi Iwayama,Tetsutaro Hayashi,Ayako Isomura-Matoba,Itoshi Nikaido,Akira Kurisaki

Nature communications 13:5878 PubMed36198679

2022

Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Aguilar,Mindaugas Pauzuolis,Malvika Pompaiah,Ehsan Vafadarnejad,Panagiota Arampatzi,Mara Fischer,Dominik Narres,Mastura Neyazi,Özge Kayisoglu,Thomas Sell,Nils Blüthgen,Markus Morkel,Armin Wiegering,Christoph-Thomas Germer,Stefan Kircher,Andreas Rosenwald,Antoine-Emmanuel Saliba,Sina Bartfeld

Oncogene 41:1507-1517 PubMed35082384

2022

De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Steiner,Gitta M Seleznik,Theresia Reding,Matea Stopic,Daniela Lenggenhager,Emiel Ten Buren,Dilmurodjon Eshmuminov,Katharina Endhardt,Catherine Hagedorn,Anna M Heidenblut,Anna Bratus-Neuenschwander,Jonas Grossmann,Christian Trachsel,Karolina S Jabbar,Stephan A Hahn,Johannes Vom Berg,Rolf Graf,Anurag Gupta

Journal of Cancer 12:4121-4133 PubMed34093815

2021

Chronic stress accelerates the process of gastric precancerous lesions in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Zheng,Weiwu Cai,Xuen Lu,Wei He,Ding Li,Haoyu Zhong,Liangjun Yang,Siyi Li,Haishan Li,Sereen Rafee,Ziming Zhao,Qi Wang,Huafeng Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GKN2 antibody [EPR15377(B)] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise