Rabbit Recombinant Monoclonal GKN2 antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications.
Images
![Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--western-blot-img105991.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15458.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)")
![Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunoprecipitation-img114763.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18370.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (AB188866)")
Publications
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- Nature communications 14:37502023Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitomi Takada,Yohei Sasagawa,Mika Yoshimura,Kaori Tanaka,Yoshimi Iwayama,Tetsutaro Hayashi,Ayako Isomura-Matoba,Itoshi Nikaido,Akira KurisakiPubMed 37386010
- Oncogene 41:1507-15172022De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina Steiner,Gitta M Seleznik,Theresia Reding,Matea Stopic,Daniela Lenggenhager,Emiel Ten Buren,Dilmurodjon Eshmuminov,Katharina Endhardt,Catherine Hagedorn,Anna M Heidenblut,Anna Bratus-Neuenschwander,Jonas Grossmann,Christian Trachsel,Karolina S Jabbar,Stephan A Hahn,Johannes Vom Berg,Rolf Graf,Anurag GuptaPubMed 35082384
- Journal of Cancer 12:4121-41332021Chronic stress accelerates the process of gastric precancerous lesions in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayi Zheng,Weiwu Cai,Xuen Lu,Wei He,Ding Li,Haoyu Zhong,Liangjun Yang,Siyi Li,Haishan Li,Sereen Rafee,Ziming Zhao,Qi Wang,Huafeng PanPubMed 34093815
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 - 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 - 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Alternative names
BLOT, GDDR, TFIZ1, UNQ465/PRO813, GKN2, Gastrokine-2, Blottin, Down-regulated in gastric cancer, Trefoil factor interactions(z) 1
Recommended products
Rabbit Recombinant Monoclonal GKN2 antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15377(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gastrokine 2 commonly known as GKN2 is a protein involved in maintaining gastric mucosal integrity. It has an approximate molecular weight of 21 kDa. GKN2 is mainly expressed in the stomach lining particularly in gastric epithelial cells. Alternate names for GKN2 include BRICD1B and CA11ORF18. By contributing to the mucus layer GKN2 helps to protect the underlying tissues from digestive acids and enzymes.
- Biological function summary
The role of GKN2 includes important activities within the gastric mucosa. It is known to form part of a protective complex in the stomach that maintains epithelial homeostasis. GKN2 interacts with other gastrokines like GKN1 to support mucosal repair and cell proliferation. It promotes gastric epithelial cell differentiation and inhibits apoptosis which helps control the integrity and function of the stomach lining.
- Pathways
GKN2 becomes integral in gastric epithelial cell signaling and repair. It associates closely with pathways related to cell cycle regulation and inflammation control within the gastric environment. GKN1 a related protein often co-regulates these processes alongside GKN2. Their interaction supports gastric healing processes reducing unnecessary cell loss and improving mucosal resilience.
- Associated diseases and disorders
GKN2 shows involvement in gastric cancer and gastritis. Alterations in GKN2 expression can indicate a disruption in gastric mucosal defenses often observed in gastric cancer development. In gastroduodenal disorders like gastritis low levels of GKN2 can correlate with increased inflammation and epithelial damage. GKN1 also shows significant connection to these diseases by interacting with GKN2 to modulate protective mechanisms in the gastric environment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--western-blot-img105991.jpg "Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)")
Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)
All lanes: Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866) at 1/5000 dilution
Lane 1: Human stomach lysate at 20 µg
Lane 2: Mouse stomach lysate at 20 µg
Lane 3: Rat stomach lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15458.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab188866 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunoprecipitation-img114763.jpg "Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)")
Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)
Western blot analysis of GKN2 in Human stomach lysate immunoprecipitated using ab188866 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)
Predicted band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866), expandable thumbnail](https://content.abcam.com/products/images/gkn2-antibody-epr15377b-c-terminal-ab188866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18370.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GKN2 with ab188866 at 1/4,000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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