Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human GLB1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.
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- Cell and tissue research 391:545-5602022CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Chen,Ling Pan,Yuexi Zeng,Xiaonan Zhu,Li YouPubMed 36525128
- iScience 25:1045672022Pro-α-cell-derived β-cells contribute to β-cell neogenesis induced by antagonistic glucagon receptor antibody in type 2 diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaona Cui,Jin Feng,Tianjiao Wei,Liangbiao Gu,Dandan Wang,Shan Lang,Kun Yang,Jin Yang,Hai Yan,Rui Wei,Tianpei HongPubMed 35789836
- Aging cell 19:e131742020Mybl2 rejuvenates heart explant-derived cells from aged donors after myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGhazaleh Rafatian et. al.PubMed 32558221
- eLife 9:2020p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Che et. al.PubMed 32125276
- Bioscience reports 39:2019Overexpressed vascular endothelial growth factor in adipose derived stem cells attenuates fibroblasts and skin injuries by ultraviolet radiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyuan Xie et. al.PubMed 31266813
- Aging 11:4145-41582019Intestinal stem cells acquire premature senescence and senescence associated secretory phenotype concurrent with persistent DNA damage after heavy ion radiation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantosh Kumar,Shubhankar Suman,Albert J Fornace,Kamal DattaPubMed 31239406
- Molecular cancer research : MCR 16:361-3772018Dual Inhibition of CDK4 and CDK2 via Targeting p27 Tyrosine Phosphorylation Induces a Potent and Durable Response in Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriyank Patel et. al.PubMed 29330290
- Aging 8:1294-3152016Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells.Applications:IHC-FrReactive species:MouseBrandon M Hall,Vitaly Balan,Anatoli S Gleiberman,Evguenia Strom,Peter Krasnov,Lauren P Virtuoso,Elena Rydkina,Slavoljub Vujcic,Karina Balan,Ilya Gitlin,Katerina Leonova,Alexander Polinsky,Olga B Chernova,Andrei V GudkovPubMed 27391570
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GLB1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P16278
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Predicted | Expected |
| Mouse | Tested | Expected |
| Rat | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Use at 1/50 - 1/200 with fluorescent detection methods. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
Alternative names
ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase
Recommended products
Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human GLB1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GLB1 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P16278
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.
- Biological function summary
GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.
- Pathways
Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.
- Associated diseases and disorders
GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (ab203749)
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse embryo tissue labeling GLB1/Beta-galactosidase using ab203749 at 1/200 dilution, followed by secondary detection and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (ab203749)
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse liver tissue labeling GLB1/Beta-galactosidase using ab203749 at 1/200 dilution, followed by secondary detection and DAB staining.
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