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AB96239

Anti-GLB1/Beta-galactosidase antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (AB96239)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (AB96239)

ab96239 staining GLB1/Beta-galactosidase in human ovarian carcinoma by immunohistochemical analysis.

Western blot - Anti-GLB1/Beta-galactosidase antibody (AB96239)
  • WB

Supplier Data

Western blot - Anti-GLB1/Beta-galactosidase antibody (AB96239)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-GLB1/Beta-galactosidase antibody (ab96239) at 1/1000 dilution

Lane 1:

A549 cell lysates at 30 µg

Lane 2:

H1299 cell lysates at 30 µg

Lane 3:

HCT116 cell lysates at 30 µg

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.
Biological function summary

GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.

Pathways

Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.

GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 17:1893-1909 PubMed31239287

2019

Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Domingo-Vidal,Diana Whitaker-Menezes,Cristina Martos-Rus,Patrick Tassone,Christopher M Snyder,Madalina Tuluc,Nancy Philp,Joseph Curry,Ubaldo Martinez-Outschoorn

Seminars in oncology 42:909-14 PubMed26615135

2015

Parathyroid Hormone-Related Peptide-Linked Hypercalcemia in a Melanoma Patient Treated With Ipilimumab: Hormone Source and Clinical and Metabolic Correlates.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Anne Mills,Marlana Orloff,Marina Domingo-Vidal,Paolo Cotzia,Ruth C Birbe,Rossitza Draganova-Tacheva,Maria P Martinez Cantarin,Madalina Tuluc,Ubaldo Martinez-Outschoorn

Seminars in oncology 40:403-18 PubMed23972703

2013

Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome?

Applications

Unspecified application

Species

Unspecified reactive species

Ubaldo E Martinez-Outschoorn,Diana Whitaker-Menezes,Matias Valsecchi,Maria P Martinez-Cantarin,Alina Dulau-Florea,Jerald Gong,Anthony Howell,Neal Flomenberg,Richard G Pestell,John Wagner,Cecilia Arana-Yi,Manish Sharma,Federica Sotgia,Michael P Lisanti

Cell cycle (Georgetown, Tex.) 11:2285-302 PubMed22684298

2012

Autophagy and senescence in cancer-associated fibroblasts metabolically supports tumor growth and metastasis via glycolysis and ketone production.

Applications

IHC-P

Species

Human

Claudia Capparelli,Carmela Guido,Diana Whitaker-Menezes,Gloria Bonuccelli,Renee Balliet,Timothy G Pestell,Allison F Goldberg,Richard G Pestell,Anthony Howell,Sharon Sneddon,Ruth Birbe,Aristotelis Tsirigos,Ubaldo Martinez-Outschoorn,Federica Sotgia,Michael P Lisanti
View all publications
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