Name: Anti-GLB1/Beta-galactosidase antibody [EPR26120-76]
SKU: ab305174
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal GLB1/Beta-galactosidase antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

Images

Western blot - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (AB305174), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-P	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Target data

See full target information GLB1

Function

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

Alternative names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

Recommended products

Rabbit Recombinant Monoclonal GLB1/Beta-galactosidase antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26120-76
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.

Biological function summary

GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.

Pathways

Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.

Associated diseases and disorders

GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Blocking and diluting buffer and concentration: 5% NFDM/TBST Low expression: Daudi
Exposure time: 15 seconds
All lanes: Western blot - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
Lane 2: SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
Lane 3: Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate 10 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 66 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling GLB1/Beta-galactosidase with ab305174 at 1/500 (1.088 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic punctate staining in SK-BR-3 cell line.Low expression: DaudiImage was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Western blot - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
All lanes: Western blot - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174) at 1/1000 dilution
Lane 1: Human lu tissue lysate 20 μg
Lane 2: Human clolon tissue lysate 20 μg
Lane 3: Human liver tissue lysate 20 μg
Lane 4: Human testis tissue lysate 10 μg
Lane 5: Human placenta tissue lysate 10 μg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Observed band size: 66 kDa
Exposure time: 15s
Immunoprecipitation - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
GLB1/Beta-galactosidase was immunoprecipitated from 0.35 mg SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab305174 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305174 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: abAB305174 IP in SK-BR-3 whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab305174 in SK-BR-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
All lanes: Immunoprecipitation - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174) at 1/1000 dilution
Lane 1: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: SK-BR-3 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 66 kDa
Exposure time: 15s
Flow Cytometry (Intracellular) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast, Left) / SK-BR-3 (human breast adenocarcinoma epithelial cell, Right) cells labelling GLB1/Beta-galactosidase with ab305174 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control: Daudi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLB1/Beta-galactosidase with ab305174 at 1/500 (1.088 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on human liver.The section was incubated with ab305174 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunohistochemical analysis of paraffin-embedded (A) MCF-7 (human bre tissue labeling GLB1/Beta-galactosidase with ab305174 at 1/500 (1.088 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on (A) MCF-7 cell pellet and (B) SK-BR-3 cell pellet; almost no staining on (C) Daudi cell pellet.The section was incubated with ab305174 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunohistochemical analysis of paraffin-embedded Human prostate hyper tissue labeling GLB1/Beta-galactosidase with ab305174 at 1/500 (1.088 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on human prostate hyperplasia (PMID: 25876105, 28234906).The section was incubated with ab305174 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GLB1/Beta-galactosidase with ab305174 at 1/500 (1.088 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection). Positive staining on human placenta (PMID: 8383699).The section was incubated with ab305174 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody [EPR26120-76] (ab305174)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GLB1/Beta-galactosidase with ab305174 at 1/500 dilution (1.088 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. Positive staining on human breast carcinoma. The section was incubated with ab305174 for 30 mins at room temperature.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins