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Rabbit Recombinant Monoclonal GLEPP1/PTPRO antibody. Suitable for Dot, IHC-P, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047), expandable thumbnail
  • Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PmIHCWB
Human
Expected
Tested
Tested
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Possesses tyrosine phosphatase activity. Plays a role in regulating the glomerular pressure/filtration rate relationship through an effect on podocyte structure and function (By similarity).

Alternative names

Rabbit Recombinant Monoclonal GLEPP1/PTPRO antibody. Suitable for Dot, IHC-P, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28645-553

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GLEPP1/PTPRO also known as protein tyrosine phosphatase receptor type O is a receptor-type protein tyrosine phosphatase with a mass around 155 kDa. It is mainly expressed in epithelial cells like podocytes in the kidney glomerulus neurons in the brain and other tissues like liver and lung. GLEPP1/PTPRO catalyzes the removal of phosphate groups from tyrosine residues on proteins an action important for regulating protein function and signal transduction. In podocytes its activity helps maintain the filtration barrier of the kidney.

Biological function summary

GLEPP1/PTPRO participates in cellular growth differentiation and apoptosis. It forms part of the podocyte slit diaphragm complex important for maintaining the glomerular filtration function in kidneys. This protein also interacts with other cellular proteins to regulate signaling pathways that control cellular architecture. Its enzymatic activity influences cellular processes by altering the phosphorylation status of partner proteins.

Pathways

GLEPP1/PTPRO plays essential roles in the MAPK and JAK/STAT signaling pathways. Through these pathways the protein mediates cellular responses to external signals influencing processes like cell cycle progression and survival. It interacts with proteins like Jak2 in these pathways modulating their activities to maintain cellular homeostasis. This involvement emphasizes its importance in signaling impacting cellular responses to environmental changes.

Associated diseases and disorders

GLEPP1/PTPRO is linked to kidney diseases such as focal segmental glomerulosclerosis (FSGS) and certain cancers. Its dysregulation can lead to abnormal podocyte function contributing to glomerular disease. Moreover in cancer alterations in its activity might affect cancer cell behavior promoting tumor progression. The relation of GLEPP1/PTPRO with proteins like nephrin highlights its contribution to maintaining healthy kidney function and its potential role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1:2000 (0.261 ug/ml) dilution, Anti-SGLT2 antibody [EPR29167-46] ab320086 anti-SLC5A2 used at 1:1000 (0.508 ug/ml) dilution and Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 anti-Aquaporin 3 used at a 1:5000 (0.0941 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-Aquaporin 3 (gray; Opal™570) on human kidney.

    Panel B: anti-PTPRP staining glomerulus in human kidney.

    Panel C: anti-SLC5A2 staining proximal tubules in human kidney.

    Panel D: anti-Aquaporin 3 staining collecting tubules in human kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322047, Anti-SGLT2 antibody [EPR29167-46] ab320086 and Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1:2000 (0.261 ug/ml) dilution, Anti-SGLT2 antibody [EPR29167-46] ab320086 anti-SLC5A2 used at 1:1000 (0.508 ug/ml) dilution and Anti-SLC12A3 antibody [EPR27106-48] ab316191 anti-SLC12A3 used at a 1:100 (5.08 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-SLC12A3 (gray; Opal™570) on human kidney.

    Panel B: anti-PTPRP staining glomerulus in human kidney.

    Panel C: anti-SLC5A2 staining proximal tubules in human kidney.

    Panel D: anti-SLC12A3 staining distal tubules in human kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322047, Anti-SGLT2 antibody [EPR29167-46] ab320086 and Anti-SLC12A3 antibody [EPR27106-48] ab316191 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1:2000 (0.261 ug/ml) dilution, Anti-SLC12A1/NKCC2 antibody [EPR28176-90] ab313640 anti-SLC12A1 used at 1:2000 (0.253 ug/ml) dilution and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1:4000 (0.141 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRO (magenta; Opal™690), anti-SCL12A1 (green; Opal™520) and anti-Angiotensin Converting Enzyme 1 (gray; Opal™570) on mouse kidney.

    Panel B: anti-PTPRO staining glomerulus in mouse kidney.

    Panel C: anti-SCL12A1 staining distal tubules in mouse kidney.

    Panel D: anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322047, Anti-SLC12A1/NKCC2 antibody [EPR28176-90] ab313640 and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1:2000 (0.261 ug/ml) dilution, Anti-SLC12A3 antibody [EPR27106-48] ab316191 anti-SLC12A3 used at 1:2000 (0.254 ug/ml) dilution and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1:4000 (0.141 ug/ml) dilution.

    Panel A: merged staining of anti-PTPRO (magenta; Opal™690), anti-SCL12A3 (green; Opal™520) and anti-Angiotensin Converting Enzyme 1 (gray; Opal™570) on mouse kidney.

    Panel B: anti-PTPRO staining glomerulus in mouse kidney.

    Panel C: anti-SCL12A3 staining distal tubules in mouse kidney.

    Panel D: anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322047, Anti-SLC12A3 antibody [EPR27106-48] ab316191 and Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Dot blot analysis of GLEPP1/PTPRO using ab322047 at 1:1000 (0.521 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: LIF-tagged human PTPRO fragment

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047) at 1/1000 dilution

    All lanes: LIF-tagged human PTPRO fragment at 20 ng

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human liver.

    The section was incubated with ab322047 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human pancreas.

    The section was incubated with ab322047 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human glomerulus.

    The section was incubated with ab322047 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse glomerulus.

    The section was incubated with ab322047 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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