AB322047

Anti-GLEPP1/PTPRO antibody [EPR28645-553]

BOND RX™ Validated
Recombinant
Advanced Validation
RabMAb
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Rabbit Recombinant Monoclonal GLEPP1/PTPRO antibody. Suitable for Dot, IHC-P, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

GLEPP1, PTPU2, PTPRO, Receptor-type tyrosine-protein phosphatase O, R-PTP-O, Glomerular epithelial protein 1, Protein tyrosine phosphatase U2, PTP-U2, PTPase U2

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human pancreas.
The section was incubated with ab322047 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

mIHC

Lab

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in human kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab199975 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab320086 anti-SLC5A2 used at 1 : 1000 (0.508 ug/ml) dilution and ab307969 anti-Aquaporin 3 used at a 1 : 5000 (0.0941 ug/ml) dilution.

Panel A : merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-Aquaporin 3 (gray; Opal™570) on human kidney.
Panel B : anti-PTPRP staining glomerulus in human kidney.
Panel C : anti-SLC5A2 staining proximal tubules in human kidney.
Panel D : anti-Aquaporin 3 staining collecting tubules in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab320086 and ab307969 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab320086 anti-SLC5A2 used at 1 : 1000 (0.508 ug/ml) dilution and ab316191 anti-SLC12A3 used at a 1 : 100 (5.08 ug/ml) dilution.

Panel A : merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-SLC12A3 (gray; Opal™570) on human kidney.
Panel B : anti-PTPRP staining glomerulus in human kidney.
Panel C : anti-SLC5A2 staining proximal tubules in human kidney.
Panel D : anti-SLC12A3 staining distal tubules in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab320086 and ab316191 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.
The section was incubated with ab322047 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

mIHC

Lab

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab273021 anti-V2R used at 1/100 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-V2R (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in human kidney.

Panel C : anti-V2R staining connecting ducts in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab273021 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human glomerulus.
The section was incubated with ab322047 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab313640 anti-SLC12A1 used at 1 : 2000 (0.253 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.141 ug/ml) dilution.

Panel A : merged staining of anti-PTPRO (magenta; Opal™690), anti-SCL12A1 (green; Opal™520) and anti-Angiotensin Converting Enzyme 1 (gray; Opal™570) on mouse kidney.
Panel B : anti-PTPRO staining glomerulus in mouse kidney.
Panel C : anti-SCL12A1 staining distal tubules in mouse kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab313640 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in mouse kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in mouse kidney.

Panel D : anti-Renin staining juxtaglomerular cells in mouse kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GLEPP1/PTPRO with ab322047 at 1/2000 (0.261 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse glomerulus.
The section was incubated with ab322047 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab316191 anti-SLC12A3 used at 1 : 2000 (0.254 ug/ml) dilution and ab254222 anti-Angiotensin Converting Enzyme 1 used at a 1 : 4000 (0.141 ug/ml) dilution.

Panel A : merged staining of anti-PTPRO (magenta; Opal™690), anti-SCL12A3 (green; Opal™520) and anti-Angiotensin Converting Enzyme 1 (gray; Opal™570) on mouse kidney.
Panel B : anti-PTPRO staining glomerulus in mouse kidney.
Panel C : anti-SCL12A3 staining distal tubules in mouse kidney.
Panel D : anti-Angiotensin Converting Enzyme 1 staining proximal tubules in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab316191 and ab254222 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Supplier Data

Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (AB322047)

Dot blot analysis of GLEPP1/PTPRO using ab322047 at 1 : 1000 (0.521 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : LIF-tagged human PTPRO fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-GLEPP1/PTPRO antibody [EPR28645-553] (ab322047) at 1/1000 dilution

All lanes:

LIF-tagged human PTPRO fragment at 20 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28645-553

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

mIHC, Dot, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/2000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/2000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLEPP1/PTPRO also known as protein tyrosine phosphatase receptor type O is a receptor-type protein tyrosine phosphatase with a mass around 155 kDa. It is mainly expressed in epithelial cells like podocytes in the kidney glomerulus neurons in the brain and other tissues like liver and lung. GLEPP1/PTPRO catalyzes the removal of phosphate groups from tyrosine residues on proteins an action important for regulating protein function and signal transduction. In podocytes its activity helps maintain the filtration barrier of the kidney.

Biological function summary

GLEPP1/PTPRO participates in cellular growth differentiation and apoptosis. It forms part of the podocyte slit diaphragm complex important for maintaining the glomerular filtration function in kidneys. This protein also interacts with other cellular proteins to regulate signaling pathways that control cellular architecture. Its enzymatic activity influences cellular processes by altering the phosphorylation status of partner proteins.

Pathways

GLEPP1/PTPRO plays essential roles in the MAPK and JAK/STAT signaling pathways. Through these pathways the protein mediates cellular responses to external signals influencing processes like cell cycle progression and survival. It interacts with proteins like Jak2 in these pathways modulating their activities to maintain cellular homeostasis. This involvement emphasizes its importance in signaling impacting cellular responses to environmental changes.

GLEPP1/PTPRO is linked to kidney diseases such as focal segmental glomerulosclerosis (FSGS) and certain cancers. Its dysregulation can lead to abnormal podocyte function contributing to glomerular disease. Moreover in cancer alterations in its activity might affect cancer cell behavior promoting tumor progression. The relation of GLEPP1/PTPRO with proteins like nephrin highlights its contribution to maintaining healthy kidney function and its potential role in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possesses tyrosine phosphatase activity. Plays a role in regulating the glomerular pressure/filtration rate relationship through an effect on podocyte structure and function (By similarity).

See full target information PTPRO

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com