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AB327139

Anti-GLI1 Antibody [CL12193]

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Mouse Monoclonal GLI1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human GLI1.

View Alternative Names

GLI, GLI1, Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-GLI1 Antibody [CL12193] (AB327139)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunofluorescence staining of RH-30 cells using ab327139 at 2–10 µg/ml, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunohistochemical staining of human oligodendroglioma using ab327139 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in a subset of tumor cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunohistochemical staining of human testis using ab327139 at 1 : 500–1 : 1000 dilution, showing moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunohistochemical staining of human cerebellum using ab327139 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in Purkinje cells, as well as in neurons in molecular layer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunohistochemical staining of mouse cerebellum using ab327139 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in Purkinje cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLI1 Antibody [CL12193] (AB327139)

Immunohistochemical staining of human liver using ab327139 at 1 : 500–1 : 1000 dilution, showing no positivity in hepatocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL12193

Isotype

IgG2b

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human GLI1.

P08151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator (PubMed : 10806483, PubMed : 19706761, PubMed : 19878745, PubMed : 24076122, PubMed : 24217340, PubMed : 24311597). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed : 2105456, PubMed : 24217340, PubMed : 8378770). Regulates the transcription of specific genes during normal development (PubMed : 19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed : 19706761, PubMed : 28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed : 11238441, PubMed : 28973407).. Isoform 2. Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
See full target information GLI1
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Product promise

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