JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240088

Anti-Gli1 antibody [EPR4523] - BSA and Azide free

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal GLI1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

GLI, GLI1, Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI

2 Images
Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088)
  • WB

Supplier Data

Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088)

This data was developed using ab134906, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The expression level of Gli1 in HepG2 and RD are consistent with describes in PMID : 19189974 and PMID : 32493277.

Negative control : HepG2 and RD (PMID : 19189974 and PMID : 32493277).

All lanes:

Western blot - Anti-Gli1 antibody [EPR4523] (<a href='/en-us/products/primary-antibodies/gli1-antibody-epr4523-ab134906'>ab134906</a>) at 1/1000 dilution

Lane 1:

A673 (human muscle Ewing's Sarcoma) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

RD (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa

Observed band size: 160 kDa

true

Exposure time: 180s

Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088)
  • WB

Supplier Data

Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088)

This data was developed using ab134906, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Gli1 antibody [EPR4523] (<a href='/en-us/products/primary-antibodies/gli1-antibody-epr4523-ab134906'>ab134906</a>) at 1/1000 dilution

Lane 1:

RMS 13 (human rhabdomyosarcoma fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

RMS 13 (human rhabdomyosarcoma fibroblast) transfected with siRNA specifically targeting Gli1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa

Observed band size: 150 kDa

true

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4523

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB53715

Anti-Patched / PTCH1 antibody

Western blot - Anti-Patched / PTCH1 antibody (AB53715)

  • 4
  • 7
Primary Antibodies

AB53281

Anti-Sonic Hedgehog antibody [EP1190Y]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (AB53281)

  • 5
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab240088 is the carrier-free version of ab134906.

This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Gli1 protein also known as GLI family zinc finger 1 acts mechanically as a transcription factor influencing gene expression. It comprises five C2H2-type zinc fingers enabling specific DNA binding activities. Gli1 has a molecular weight of approximately 118 kDa. Expression occurs prominently in skin brain and bone tissues. As an important effector in the Hedgehog (Hh) signaling pathway Gli1 transmits signals from the cell membrane to the nucleus.
Biological function summary

Gli1 functions to regulate cellular proliferation differentiation and survival. As part of the Gli protein family Gli1 operates in concert with other members such as Gli2 and Gli3 though it lacks significant repressor activity unlike some of its counterparts. It acts prominently in developmental processes controlling the expression of genes critical for embryogenesis. Gli1 also maintains stem cell populations and sustains tissue homeostasis in adults particularly in contexts where rapid cell division is necessary like wound healing.

Pathways

Gli1 plays a substantial role in the Hedgehog signaling pathway and interacts dynamically with this system. It receives upstream activation from the Smoothened protein which conveys signals in response to Hedgehog ligand availability. Additionally the Gli1 pathway overlaps with WNT signaling during various developmental stages. Through these interactions Gli1 coordinates tightly with other proteins to effect changes in target gene transcription that influence cell fate decisions.

Aberrant Gli1 activity associates strongly with certain cancers such as basal cell carcinoma and medulloblastoma. Persistent activation of the Hedgehog pathway often due to mutations in related proteins like Patched or Smoothened leads to excessive Gli1 activation and subsequent tumorigenesis. Beyond oncology Gli1's dysregulation also connects with congenital disorders such as Gorlin syndrome. Here mutations influence a broad network of genes that often include members of the Gli family causing developmental anomalies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a transcriptional activator (PubMed : 10806483, PubMed : 19706761, PubMed : 19878745, PubMed : 24076122, PubMed : 24217340, PubMed : 24311597). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed : 2105456, PubMed : 24217340, PubMed : 8378770). Regulates the transcription of specific genes during normal development (PubMed : 19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed : 19706761, PubMed : 28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed : 11238441, PubMed : 28973407).. Isoform 2. Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
See full target information GLI1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 14:2526444 PubMed40891421

2025

Chronic antigen stimulation in melanoma induces T cell exhaustion and limits efficacy of T cell bispecific therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Idil Hutter-Karakoc,Eleni Maria Varypataki,Aparna Neelakandhan,Simone Lang,Vesna Kramar,Ahmet Varol,Sasha Simons,Marine Richard,Mudita Pincha,Dario Venetz,Johannes Sam,Nicole Joller,Christian Münz,Pablo Umana,Christian Klein,Maria Amann

Cell death & disease 11:358 PubMed32398664

2020

CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Yangke He,Hui Huang,Li Jin,Fang Zhang,Ming Zeng,Liang Wei,Shijia Tang,Dongqin Chen,Wansheng Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com