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Rabbit Recombinant Monoclonal GLI1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088), expandable thumbnail
  • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088), expandable thumbnail
  • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088), expandable thumbnail
  • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (AB240088), expandable thumbnail

Publications

  • Cell death & disease 11:3582020
    CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yangke He et. al.
    PubMed 32398664

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    1 products for Alternative Product

      Target data

      Function

      Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). Regulates the transcription of specific genes during normal development (PubMed:19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407).Isoform 2Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

      Alternative names

      Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI

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      Rabbit Recombinant Monoclonal GLI1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

      Alternative names

      Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR4523

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab240088 is the carrier-free version of Anti-Gli1 antibody [EPR4523] ab134906.

      This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      The Gli1 protein also known as GLI family zinc finger 1 acts mechanically as a transcription factor influencing gene expression. It comprises five C2H2-type zinc fingers enabling specific DNA binding activities. Gli1 has a molecular weight of approximately 118 kDa. Expression occurs prominently in skin brain and bone tissues. As an important effector in the Hedgehog (Hh) signaling pathway Gli1 transmits signals from the cell membrane to the nucleus.

      Biological function summary

      Gli1 functions to regulate cellular proliferation differentiation and survival. As part of the Gli protein family Gli1 operates in concert with other members such as Gli2 and Gli3 though it lacks significant repressor activity unlike some of its counterparts. It acts prominently in developmental processes controlling the expression of genes critical for embryogenesis. Gli1 also maintains stem cell populations and sustains tissue homeostasis in adults particularly in contexts where rapid cell division is necessary like wound healing.

      Pathways

      Gli1 plays a substantial role in the Hedgehog signaling pathway and interacts dynamically with this system. It receives upstream activation from the Smoothened protein which conveys signals in response to Hedgehog ligand availability. Additionally the Gli1 pathway overlaps with WNT signaling during various developmental stages. Through these interactions Gli1 coordinates tightly with other proteins to effect changes in target gene transcription that influence cell fate decisions.

      Associated diseases and disorders

      Aberrant Gli1 activity associates strongly with certain cancers such as basal cell carcinoma and medulloblastoma. Persistent activation of the Hedgehog pathway often due to mutations in related proteins like Patched or Smoothened leads to excessive Gli1 activation and subsequent tumorigenesis. Beyond oncology Gli1's dysregulation also connects with congenital disorders such as Gorlin syndrome. Here mutations influence a broad network of genes that often include members of the Gli family causing developmental anomalies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      4 product images

      • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088), expandable thumbnail

        Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)

        This data was developed using Anti-Gli1 antibody [EPR4523] ab134906, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-Gli1 antibody [EPR4523] (Anti-Gli1 antibody [EPR4523] ab134906) at 1/1000 dilution

        All lanes: RMS13 cell lysate at 10 µg

        Secondary

        All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 118 kDa

        Observed band size: 150 kDa

      • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088), expandable thumbnail

        Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)

        This data was developed using Anti-Gli1 antibody [EPR4523] ab134906, the same antibody clone in a different buffer formulation.

        All lanes: Western blot - Anti-Gli1 antibody [EPR4523] (Anti-Gli1 antibody [EPR4523] ab134906) at 1/1000 dilution

        Lane 1: RMS13 cell lysate at 10 µg

        Lane 2: A673 cell lysate at 10 µg

        Secondary

        All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

        Predicted band size: 118 kDa

      • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088), expandable thumbnail

        Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)

        This data was developed using Anti-Gli1 antibody [EPR4523] ab134906, the same antibody clone in a different buffer formulation.

        Diluting and blocking buffer and concentration: 5% NFDM/TBST

        This blot was developed using a high sensitivity ECL substrate.

        All lanes: Western blot - Anti-Gli1 antibody [EPR4523] (Anti-Gli1 antibody [EPR4523] ab134906) at 1/1000 dilution

        Lane 1: RMS 13 (human rhabdomyosarcoma fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

        Lane 2: RMS 13 (human rhabdomyosarcoma fibroblast) transfected with siRNA specifically targeting Gli1 whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 118 kDa

        Observed band size: 150 kDa

        Exposure time: 180s

      • Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088), expandable thumbnail

        Western blot - Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)

        This data was developed using Anti-Gli1 antibody [EPR4523] ab134906, the same antibody clone in a different buffer formulation.

        Diluting and blocking buffer and concentration: 5% NFDM/TBST

        This blot was developed using a high sensitivity ECL substrate.

        The expression level of Gli1 in HepG2 and RD are consistent with describes in PMID: 19189974 and PMID: 32493277.

        Negative control: HepG2 and RD (PMID: 19189974 and PMID: 32493277).

        All lanes: Western blot - Anti-Gli1 antibody [EPR4523] (Anti-Gli1 antibody [EPR4523] ab134906) at 1/1000 dilution

        Lanes 1 - 2: A673 (human muscle Ewing's Sarcoma) whole cell lysate at 20 µg

        Lane 3: RD (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 118 kDa

        Observed band size: 160 kDa

        Exposure time: 180s

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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