JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB187386

Anti-Gli2 antibody [OTI1F9]

Be the first to review this product! Submit a review

|

(11 Publications)

Mouse Monoclonal GLI2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLI2 aa 700-1150.

View Alternative Names

THP, GLI2, Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli2 antibody [OTI1F9] (AB187386)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli2 antibody [OTI1F9] (AB187386)

Paraffin-embedded Human skin tissue labeling Gli2 with ab187386 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-Gli2 antibody [OTI1F9] (AB187386)
  • WB

Supplier Data

Western blot - Anti-Gli2 antibody [OTI1F9] (AB187386)

All lanes:

Western blot - Anti-Gli2 antibody [OTI1F9] (ab187386) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA at 5 µg

Predicted band size: 102 kDa,167 kDa,70 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1F9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GLI2 aa 700-1150. The exact immunogen used to generate this antibody is proprietary information.

P10070

style
left-column

Complementary Products

Primary Antibodies

AB134906

Anti-Gli1 antibody [EPR4523]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Gli1 antibody [EPR4523] (AB134906)

  • 3
  • 2
Primary Antibodies

AB53281

Anti-Sonic Hedgehog antibody [EP1190Y]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (AB53281)

  • 5
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB53715

Anti-Patched / PTCH1 antibody

Western blot - Anti-Patched / PTCH1 antibody (AB53715)

  • 4
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gli2 also known as GLI family zinc finger 2 is a transcription factor with a molecular mass of approximately 160 kDa. It is an important component of the Hedgehog (Hh) signaling pathway. Gli2 primarily resides in the cytoplasm and migrates to the nucleus upon activation. Expression of Gli2 is widespread but most notable in developing tissues and adult brain. In the nucleus it regulates transcription by binding to specific DNA sequences.
Biological function summary

Gli2 acts as an activator or repressor of gene expression depending on the cellular context. It forms part of the Gli family of transcription factors playing a significant role in cellular growth differentiation and tissue patterning. While not usually part of stable protein complexes Gli2 interacts transiently with other proteins such as Suppressor of Fused (SuFu) to regulate its transcriptional activity. This interaction influences the processing and degradation of Gli proteins.

Pathways

The Hedgehog signaling cascade prominently features Gli2 as a critical mediator. This pathway is fundamental in embryonic development. Alongside other Gli proteins like Gli1 and Gli3 Gli2 helps to translate extracellular Hh signals into precise cellular responses. In the absence of Hh ligands Gli2 remains tethered in the cytoplasm in a complex with SuFu limiting its transcription activity. Upon Hh pathway activation Gli2 translocates to the nucleus and modulates target gene expression.

Gli2 has a notable connection to cancers including basal cell carcinoma and medulloblastoma. Overactive Hedgehog signaling often involves dysregulation of Gli2 which promotes tumorigenesis by driving inappropriate cellular proliferation. Additionally Gli2 links to the genetic disorder Greig cephalopolysyndactyly syndrome via its interactions with Gli3 protein. These connections underline the importance of Gli2 as a therapeutic target in certain pathological conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a transcription regulator in the hedgehog (Hh) pathway (PubMed : 18455992, PubMed : 26565916). Functions as a transcriptional activator (PubMed : 19878745, PubMed : 24311597, PubMed : 9557682). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed : 15994174, PubMed : 20685856).. Isoform 1. Involved in the smoothened (SHH) signaling pathway.. Isoform 2. Involved in the smoothened (SHH) signaling pathway.. Isoform 3. Involved in the smoothened (SHH) signaling pathway.. Isoform 4. Involved in the smoothened (SHH) signaling pathway.. Isoform 1. Acts as a transcriptional activator in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 2. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 3. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 4. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 5. Acts as a transcriptional repressor.
See full target information GLI2
style
left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1267 PubMed39894896

2025

The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shahid Banday,Alok K Mishra,Romana Rashid,Tianyi Ye,Amjad Ali,Junhui Li,Jason T Yustein,Michelle A Kelliher,Lihua Julie Zhu,Sara K Deibler,Sunil K Malonia,Michael R Green

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Technology in cancer research & treatment 23:15330338241274337 PubMed39166273

2024

Tumor-Associated Macrophage-Derived TGF-β1 Activates GLI2 via the Smad2/3 Signaling Pathway to Affect Cisplatin Resistance in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Lan,Dalong Wei,Lini Fang,Xiangsheng Wu,Biaoliang Wu

Anticancer research 43:2909-2922 PubMed37351966

2023

MAML3 Contributes to Induction of Malignant Phenotype of Gallbladder Cancer Through Morphogenesis Signalling Under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Na,Hideya Onishi,Shinji Morisaki,Shu Ichimiya,Yutaka Yamada,Shogo Masuda,Shinjiro Nagao,Satoko Koga,Kazunori Nakayama,Akira Imaizumi,Yoshinao Oda,Masafumi Nakamura

Cell biology international 46:1458-1467 PubMed35579188

2022

MUC21 controls melanoma progression via regulating SLITRK5 and hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xueping Liu,Yajun Xiao,Xia Xiong,Xiaoyi Qi

Aging 13:10749-10769 PubMed33848981

2021

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Peng,Xiaojing Zhang,Huijuan Lin,Shiqi Deng,Ying Qin,Jieqiong He,Fan Hu,Xiaohui Zhu,Xianling Feng,Jian Wang,Yanjie Wei,Xinmin Fan,Huan Lin,Hassan Ashktorab,Duane Smoot,Yansi Lv,Song Li,Stephen J Meltzer,Zhe Jin

Oncology reports 45:997-1010 PubMed33650666

2021

GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Ichimiya,Hideya Onishi,Shinjiro Nagao,Satoko Koga,Kukiko Sakihama,Kazunori Nakayama,Akiko Fujimura,Yasuhiro Oyama,Akira Imaizumi,Yoshinao Oda,Masafumi Nakamura

Experimental and molecular pathology 116:104492 PubMed32668286

2020

Circ_DCAF6 potentiates cell stemness and growth in breast cancer through GLI1-Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guolin Ye,Ruilin Pan,Leiwei Zhu,Dan Zhou

Molecules and cells 42:661-671 PubMed31564076

2019

Small Molecule-Based Strategy Promotes Nucleus Pulposus Specific Differentiation of Adipose-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Hua,Ning Shen,Jingkai Wang,Yiqing Tao,Fangcai Li,Qixin Chen,Xiaopeng Zhou

Frontiers in pharmacology 10:1039 PubMed31616295

2019

Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yan Zhang,Zhong-Min Jiang,Xiao-Fang Ma,Yue Li,Xiao-Zhi Liu,Li-Li Li,Wen-Han Wu,Tao Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com