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AB6050

Anti-Gli3 antibody

3

(3 Reviews)

|

(23 Publications)

Rabbit Polyclonal GLI3 antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human GLI3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody (AB6050)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody (AB6050)

ICC/IF image of ab6050 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6050, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody (AB6050)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody (AB6050)

ab6050 at 0.625 μg/ml staining human glioblastoma.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB, IHC-Fr, IHC-P

applications

Immunogen

Synthetic Peptide within Human GLI3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10071

Specificity

A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity-purified antibody is directed against human Gli-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.
Biological function summary

Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.

Pathways

Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.

Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
See full target information GLI3
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35041619

2022

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via sonic hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hairong Shi,Hongshun Wang,Cheng Zhang,Yajie Lu,Jun Yao,Zhibin Chen,Guangqian Xing,Qinjun Wei,Xin Cao

BioMed research international 2021:8889986 PubMed33506047

2021

GLI3 Promotes Invasion and Predicts Poor Prognosis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Shen,Zhengyuan Zhang,Ping Wang

Experimental & molecular medicine 53:52-66 PubMed33390589

2021

Dysfunction of Shh signaling activates autophagy to inhibit trophoblast motility in recurrent miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Yibin Pan,Lili Yan,Qiaoqiao Chen,Cheng Wei,Yongdong Dai,Xiaomei Tong,Haiyan Zhu,Meifei Lu,Yanling Zhang,Xiaoying Jin,Tai Zhang,Xiaona Lin,Feng Zhou,Songying Zhang

Frontiers in genetics 11:542004 PubMed33304378

2020

A Novel Nonsense Variant Is Associated With Polydactyly and Syndactyly in a Family by Blocking the Sonic Hedgehog Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xiang,Xiaoliang Li,Zhiyan Zhan,Jue Feng,Haiqing Cai,Yanxin Li,Qihua Fu,Yunlan Xu,Hong Jiang,Xiaoqing Zhang

Cancer management and research 12:3903-3914 PubMed32547226

2020

Long Noncoding RNA DIO3OS Hinders Cell Malignant Behaviors of Hepatocellular Carcinoma Cells Through the microRNA-328/Hhip Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanpeng Wang,Lina Song,Yanshuo Ye,Wei Li

Cell death & disease 11:358 PubMed32398664

2020

CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Yangke He,Hui Huang,Li Jin,Fang Zhang,Ming Zeng,Liang Wei,Shijia Tang,Dongqin Chen,Wansheng Wang

Molecular medicine reports 21:1861-1871 PubMed32319630

2020

Gli3 is a novel downstream target of miR‑200a with an anti‑fibrotic role for progression of liver fibrosis in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Jianghua Ran,Lan Li,Gang Chen,Shengning Zhang,Yingjia Wang

Frontiers in pharmacology 10:1039 PubMed31616295

2019

Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yan Zhang,Zhong-Min Jiang,Xiao-Fang Ma,Yue Li,Xiao-Zhi Liu,Li-Li Li,Wen-Han Wu,Tao Wang

Oncogenesis 7:97 PubMed30563983

2018

miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Yan Liu,Jian Guo,Huiwen He,Xue Mi,Chong Chen,Junling Xie,Shengnan Wang,Peng Wu,Fengqi Cao,Lipeng Bai,Qin Si,Rong Xiang,Yunping Luo

Journal of bone and mineral metabolism 37:594-606 PubMed30470957

2018

Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Yu,Dong Li,Dan Wang,Chun-Mei Hu,Yan Sun,Yan Tang,Guang Shi
View all publications
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