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Rabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

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Images

Western blot - Anti-Gli3 antibody [EPR26837-58] (AB307714), expandable thumbnail
  • Western blot - Anti-Gli3 antibody [EPR26837-58] (AB307714), expandable thumbnail
  • Immunoprecipitation - Anti-Gli3 antibody [EPR26837-58] (AB307714), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PIHC-FrICC/IF
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

Alternative names

Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, Gli3

Recommended products

Rabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26837-58
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.

Biological function summary

Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.

Pathways

Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.

Associated diseases and disorders

Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714), expandable thumbnail

    Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody can detect the GLI3 full-length protein (190 kDa) and GLI3 C-terminally truncated form (83 kDa), which is consistent with that described in the literature (PMID: 20360384; PMID: 31554934).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: NIH/3T3 transfected with siRNA specifically targeti GLI3 whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 83 kDa, 190 kDa

    Exposure time: 48s

  • Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714), expandable thumbnail

    Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody can detect the GLI3 full-length protein (190 kDa) and GLI3 C-terminally truncated form (83 kDa), which is consistent with that described in the literature (PMID: 20360384; PMID: 31554934).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.


    Exposure time: 180 seconds

    All lanes: Western blot - Anti-Gli3 antibody [EPR26837-58] (ab307714) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 83 kDa, 190 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-Gli3 antibody [EPR26837-58] (ab307714), expandable thumbnail

    Immunoprecipitation - Anti-Gli3 antibody [EPR26837-58] (ab307714)

    Gli3 was immunoprecipitated from 0.35 mg NIH/3T3 whole cell lysate with ab307714 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307714 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2: ab307714 IP in NIH/3T3 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307714 in NIH/3T3 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 32 seconds
    The lysate was freshly made and used for IP immediately to minimize protein degradation.

    All lanes: Immunoprecipitation - Anti-Gli3 antibody [EPR26837-58] (ab307714) at 1/30 dilution

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 32s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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