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Rabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130), expandable thumbnail
  • Western blot - Anti-Gli3 antibody [EPR4594] (AB181130), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Chimpanzee
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Chimpanzee
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

Alternative names

Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI3

Recommended products

Rabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4594
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.

Biological function summary

Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.

Pathways

Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.

Associated diseases and disorders

Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail
    This image is courtesy of a customer review by Lindsey Fitzsimons

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x100.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x40.

  • Western blot - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail

    Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)

    All lanes: Western blot - Anti-Gli3 antibody [EPR4594] (ab181130) at 1/1000 dilution

    All lanes: 293 lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 170 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail
    This image is courtesy of a customer review submitted by Lindsey Fitzsimons

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (ab181130)

    Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed Human Kidney, FFPE cortex/medulla tissue. Stained with ab181130 at 1/100 dilution. Secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078 at 1/1000 dilution. Blocking was done with 5% serum and 0.3% Triton X-100 for 30 minutes at 20°C. The sample was incubated with the primary antibody and 1XPBS with 1%BSA and 0.3% Triton X-100 for 10 hours at 4°C.

  • Western blot - Anti-Gli3 antibody [EPR4594] (ab181130), expandable thumbnail

    Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)

    Freshly made lysate is highly recommended to get desired WB result

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Gli3 antibody [EPR4594] (ab181130) at 1/400 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 170 kDa

    Observed band size: 190 kDa

    Exposure time: 100s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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