AB181130

Anti-Gli3 antibody [EPR4594]

Name: Anti-Gli3 antibody [EPR4594] (ab181130) | Abcam
Brand: Abcam Limited
SKU: AB181130
Price: 225 USD
Availability: InStock
Rating: 5 (4 reviews)

RabMAb
Recombinant
What is this?

(4 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI3

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 (green) with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin (1/1000). Magnification at x40.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130)

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130)

This image is courtesy of an abreview by Lindsey Fitzsimons

IHC-P

AbReview75541****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed Human Kidney, FFPE cortex/medulla tissue. Stained with ab181130 at 1/100 dilution. Secondary antibody used was ab150078 at 1/1000 dilution. Blocking was done with 5% serum and 0.3% Triton X-100 for 30 minutes at 20°C. The sample was incubated with the primary antibody and 1XPBS with 1%BSA and 0.3% Triton X-100 for 10 hours at 4°C.

This image is courtesy of an Abreview submitted by Lindsey Fitzsimons

Supplier Data

Western blot - Anti-Gli3 antibody [EPR4594] (AB181130)

All lanes:

Western blot - Anti-Gli3 antibody [EPR4594] (ab181130) at 1/1000 dilution

All lanes:

293 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 170 kDa

false

Lab

Western blot - Anti-Gli3 antibody [EPR4594] (AB181130)

Freshly made lysate is highly recommended to get desired WB result Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Gli3 antibody [EPR4594] (ab181130) at 1/400 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 170 kDa

Observed band size: 190 kDa

false

Exposure time: 100s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4594

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.

Biological function summary

Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.

Pathways

Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.

Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

See full target information GLI3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of reproduction and development 71:161-167 PubMed40301068

2025

Mechanism of action of IHH in ameliorating thin endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Luo,Man Luo,Donghong Ning,Xi Chen,Qiuman Zheng,Qin Cao

Developmental dynamics : an official publication of the American Association of Anatomists 253:312-332 PubMed37776236

2023

Primary cilia are critical for tracheoesophageal septation.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey Avery Fitzsimons,Evangelia Tasouri,Marc August Willaredt,Daniel Stetson,Christian Gojak,Joachim Kirsch,Humphrey A R Gardner,Karin Gorgas,Kerry L Tucker

Cell regeneration (London, England) 12:3 PubMed36720733

2023

Hippo signaling activates hedgehog signaling by Taz-driven Gli3 processing.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Tang,Jirong Wang,Minli Yao,Xing Ji,Wei Shi,Chengyun Xu,Ling-Hui Zeng,Ximei Wu

Scientific reports 12:14173 PubMed35986080

2022

Altered sialidase expression in human myeloid cells undergoing apoptosis and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang W Hyun,Chiguang Feng,Anguo Liu,Erik P Lillehoj,Rossana Trotta,Tami J Kingsbury,Antonino Passaniti,Katerina N Lugkey,Sitara Chauhan,John F Cipollo,Irina G Luzina,Sergei P Atamas,Alan S Cross,Simeon E Goldblum

Cancer science 112:2260-2271 PubMed33837611

2021

LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Pan,Jingxue Tan,Tao Tao,Xiuwen Zhang,Yiping Weng,Xiaokun Weng,Jingwen Xu,Haibo Li,Yuqing Jiang,Dong Zhou,Yifei Shen

OncoTargets and therapy 13:11957-11973 PubMed33239895

2020

Histone Demethylase Promotes Cervical Cancer Malignancy Through the /KIF14/Hedgehog Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Liu,Dongqing Li,Xin Zhang,Yanyan Li,Jian Ou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Gli3 antibody [EPR4594]

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody [EPR4594] (AB181130)

Western blot - Anti-Gli3 antibody [EPR4594] (AB181130)

Western blot - Anti-Gli3 antibody [EPR4594] (AB181130)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise