Rabbit Polyclonal Gliomedin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 7 publications.
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- The Journal of clinical investigation 132:2022Schwann cell nodal membrane disruption triggers bystander axonal degeneration in a Guillain-Barré syndrome mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRhona McGonigal,Clare I Campbell,Jennifer A Barrie,Denggao Yao,Madeleine E Cunningham,Colin L Crawford,Simon Rinaldi,Edward G Rowan,Hugh J WillisonPubMed 35671105
- Journal of anatomy 241:1259-12712021The role of gangliosides in the organisation of the node of Ranvier examined in glycosyltransferase transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRhona McGonigal,Hugh J WillisonPubMed 34605014
- Cell reports. Medicine 2:1003452021An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex J Clark,Umaiyal Kugathasan,Georgios Baskozos,David A Priestman,Nadine Fugger,Museer A Lone,Alaa Othman,Ka Hing Chu,Iulia Blesneac,Emma R Wilson,Matilde Laurà,Bernadett Kalmar,Linda Greensmith,Thorsten Hornemann,Frances M Platt,Mary M Reilly,David L BennettPubMed 34337561
- The Journal of biological chemistry 288:26357-712013Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeung Sun Kwon et. al.PubMed 23897819
- Neurology 81:714-222013Anti-neurofascin antibody in patients with combined central and peripheral demyelination.
- Neuron 69:244-572011Nodes of Ranvier act as barriers to restrict invasion of flanking paranodal domains in myelinated axons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney Thaxton,Anilkumar M Pillai,Alaine L Pribisko,Jeffrey L Dupree,Manzoor A BhatPubMed 21262464
- The Journal of neuroscience : the official journal of the Society for Neuroscience 30:4868-762010In vivo deletion of immunoglobulin domains 5 and 6 in neurofascin (Nfasc) reveals domain-specific requirements in myelinated axons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney Thaxton,Anilkumar M Pillai,Alaine L Pribisko,Marilyne Labasque,Jeffrey L Dupree,Catherine Faivre-Sarrailh,Manzoor A BhatPubMed 20371806
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Ligand for NRCAM and NFASC/neurofascin that plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Mediates interaction between Schwann cell microvilli and axons via its interactions with NRCAM and NFASC (PubMed:16039564). Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with NRCAM, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (By similarity).
Alternative names
Gliomedin, Gldn
Recommended products
Rabbit Polyclonal Gliomedin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab24483 detects a Gliomedin sized band at 70kDa in WB.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gliomedin also known by the alternate name Milk Fat Globule-EGF Factor 8 Protein or MFG-E8 plays an important role in the peripheral nervous system. It is a glycoprotein with a mass of approximately 40-50 kDa. Gliomedin expresses mainly at the node of Ranvier particularly in Schwann cells surrounding the peripheral nerves. This expression is key for maintaining proper function of myelinated nerves.
- Biological function summary
Gliomedin aids in the formation and maintenance of the nodes of Ranvier by interacting with proteins such as Neurofascin and NgCAM-related cell adhesion molecule (NrCAM). It is part of a complex which includes these proteins contributing to nodal organization and stability. Gliomedin anchors at the Schwann cell microvilli and connects with axonal cell adhesion molecules ensuring efficient signal transmission in nerve fibers.
- Pathways
Gliomedin interacts with cellular pathways responsible for nerve development and maintenance namely the axon guidance and myelin sheathing pathways. Its interaction with Neurofascin and NrCAM proteins facilitates proper node assembly and nerve signaling. These interactions are important to maintaining the fast conduction velocities required for signal transmission in the peripheral nervous system.
- Associated diseases and disorders
Alterations in gliomedin function relate to neuropathies and demyelinating diseases such as Guillain-Barré syndrome and Charcot-Marie-Tooth disease. Disruption of gliomedin interactions with Neurofascin and NrCAM can lead to compromised node integrity contributing to nerve conduction problems characteristic of these disorders. Understanding gliomedin's role may aid in developing therapeutic approaches targeting these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Gliomedin antibody (ab24483)
ab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.
All lanes: Western blot - Anti-Gliomedin antibody (ab24483) at 1 µg/mL
Lane 1: Mouse Sciatic Nerve Tissue Lysate at 10 µg
Lane 2: Rat Sciatic Nerve Tissue Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa
Exposure time: 4min
Immunocytochemistry/ Immunofluorescence - Anti-Gliomedin antibody (ab24483)
ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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