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AB252974

Anti-GlnRS antibody

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(1 Publication)

Rabbit Polyclonal GlnRS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human QARS1 aa 600-750.

View Alternative Names

QARS, QARS1, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, GlnRS

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)

Paraffin-embedded human liver tissue stained for GlnRS using ab252974 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GlnRS antibody (AB252974)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GlnRS antibody (AB252974)

PFA-fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells stained for GlnRS (green) using ab252974 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)

Paraffin-embedded human cerebral cortex tissue stained for GlnRS using ab252974 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)

Paraffin-embedded human testis tissue stained for GlnRS using ab252974 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GlnRS antibody (AB252974)

Paraffin-embedded human colon tissue stained for GlnRS using ab252974 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-GlnRS antibody (AB252974)
  • WB

Supplier Data

Western blot - Anti-GlnRS antibody (AB252974)

All lanes:

Western blot - Anti-GlnRS antibody (ab252974) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 88 kDa

false

Western blot - Anti-GlnRS antibody (AB252974)
  • WB

Supplier Data

Western blot - Anti-GlnRS antibody (AB252974)

All lanes:

Western blot - Anti-GlnRS antibody (ab252974) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human QARS1 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

P47897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminyl-tRNA synthetase (GlnRS) is an enzyme that catalyzes the attachment of glutamine to its corresponding tRNA molecule facilitating protein synthesis. Known also as QARS or GLNRS GlnRS has a molecular mass of approximately 75 kilodaltons. This enzyme is expressed in both prokaryotic and eukaryotic organisms indicating its fundamental role across diverse biological systems. In eukaryotes GlnRS is usually located in the cytoplasm where protein biosynthesis occurs. The structure of GlnRS includes an anticodon binding domain which allows it to recognize and bind specifically to tRNA^Gln.
Biological function summary

GlnRS ensures the correct translation of genetic information into proteins by attaching glutamine to the appropriate tRNA forming glutaminyl-tRNA^Gln. GlnRS operates as a monomer rather than being part of a larger protein complex. This activity is important for maintaining the fidelity of protein translation as it ensures that the amino acid sequence of the protein matches the codon sequence of the mRNA. Errors in tRNA charging can lead to the incorporation of incorrect amino acids potentially resulting in nonfunctional or deleterious proteins.

Pathways

GlnRS plays an important role in the aminoacyl-tRNA biosynthesis pathway which is integral for the translation of genetic information into functional proteins. This pathway is part of the larger translation machinery connecting genetic code with amino acid sequences. GlnRS is closely related to other aminoacyl-tRNA synthetases such as alanyl-tRNA synthetase (AlaRS) and leucyl-tRNA synthetase (LeuRS) which similarly charge their respective tRNAs. However each synthetase maintains particular specificity to ensure correct protein synthesis.

Disruptions in the function of GlnRS can relate to neurological disorders such as Charcot-Marie-Tooth disease due to its role in protein biosynthesis. Mutations in GlnRS or associated aminoacyl-tRNA synthetases like GlyRS have been documented in these disorders. Misregulation of GlnRS can lead to impaired protein synthesis and accumulation of misfolded proteins contributing to disease pathogenesis. Understanding the relationship between GlnRS and disease helps in developing potential therapeutic targets for related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glutamine--tRNA ligase (PubMed : 26869582). Plays a critical role in brain development (PubMed : 24656866).
See full target information QARS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:539 PubMed35837031

2022

Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Hiramatsu,Naoki Yamamoto,Yu Kato,Noriaki Nagai,Sumito Isogai,Kazuyoshi Imaizumi
View all publications
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Product promise

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