AB226296

Anti-GlnRS antibody - C-terminal

Name: Anti-GlnRS antibody - C-terminal (ab226296) | Abcam
Brand: Abcam Limited
SKU: AB226296

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(1 Publication)

Rabbit Polyclonal GlnRS antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human QARS1 aa 700-800.

View Alternative Names

QARS, QARS1, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, GlnRS

2 Images

Supplier Data

Immunoprecipitation - Anti-GlnRS antibody - C-terminal (AB226296)

GlnRS was immunoprecipitated from HEK-293T (human epithelial epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226296 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226296 at 0.4 μg/ml.

Lane 1 : ab226296 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-GlnRS antibody - C-terminal (ab226296)

Predicted band size: 88 kDa

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Supplier Data

Western blot - Anti-GlnRS antibody - C-terminal (AB226296)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-GlnRS antibody - C-terminal (ab226296) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 88 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human QARS1 aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

P47897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226296 was affinity purified using an epitope specific to GlnRS immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminyl-tRNA synthetase (GlnRS) is an enzyme that catalyzes the attachment of glutamine to its corresponding tRNA molecule facilitating protein synthesis. Known also as QARS or GLNRS GlnRS has a molecular mass of approximately 75 kilodaltons. This enzyme is expressed in both prokaryotic and eukaryotic organisms indicating its fundamental role across diverse biological systems. In eukaryotes GlnRS is usually located in the cytoplasm where protein biosynthesis occurs. The structure of GlnRS includes an anticodon binding domain which allows it to recognize and bind specifically to tRNA^Gln.

Biological function summary

GlnRS ensures the correct translation of genetic information into proteins by attaching glutamine to the appropriate tRNA forming glutaminyl-tRNA^Gln. GlnRS operates as a monomer rather than being part of a larger protein complex. This activity is important for maintaining the fidelity of protein translation as it ensures that the amino acid sequence of the protein matches the codon sequence of the mRNA. Errors in tRNA charging can lead to the incorporation of incorrect amino acids potentially resulting in nonfunctional or deleterious proteins.

Pathways

GlnRS plays an important role in the aminoacyl-tRNA biosynthesis pathway which is integral for the translation of genetic information into functional proteins. This pathway is part of the larger translation machinery connecting genetic code with amino acid sequences. GlnRS is closely related to other aminoacyl-tRNA synthetases such as alanyl-tRNA synthetase (AlaRS) and leucyl-tRNA synthetase (LeuRS) which similarly charge their respective tRNAs. However each synthetase maintains particular specificity to ensure correct protein synthesis.

Disruptions in the function of GlnRS can relate to neurological disorders such as Charcot-Marie-Tooth disease due to its role in protein biosynthesis. Mutations in GlnRS or associated aminoacyl-tRNA synthetases like GlyRS have been documented in these disorders. Misregulation of GlnRS can lead to impaired protein synthesis and accumulation of misfolded proteins contributing to disease pathogenesis. Understanding the relationship between GlnRS and disease helps in developing potential therapeutic targets for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Glutamine--tRNA ligase (PubMed : 26869582). Plays a critical role in brain development (PubMed : 24656866).

See full target information QARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 31:631-643 PubMed37547755

2023

The anti-oncogenic effect of 17-DMAG via the inactivation of HSP90 and MET pathway in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Kawano,Kazuhiro Tanaka,Ichiro Itonaga,Tatsuya Iwasaki,Yuta Kubota,Hiroshi Tsumura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GlnRS antibody - C-terminal

Immunoprecipitation - Anti-GlnRS antibody - C-terminal (AB226296)

Western blot - Anti-GlnRS antibody - C-terminal (AB226296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise